Cell Biology Antibodies 5

Anti-AFM Antibody (CAB15253)

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SKU:
CAB15253
Product Type:
Antibody
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-AFM Antibody
Antibody SKU:CAB15253
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 260-599 of human AFM (NP_001124.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Rat
Positive Samples:Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 260-599 of human AFM (NP_001124.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EDVS SNYD GCCE GDVV QCIR DTSK VMNH ICSK QDSI SSKI KECC EKKI PERG QCII NSNK DDRP KDLS LREG KFTD SENV CQER DADP DTFF AKFT FEYS RRHP DLSI PELL RIVQ IYKD LLRN CCNT ENPP GCYR YAED KFNE TTEK SLKM VQQE CKHF QNLG KDGL KYHY LIRL TKIA PQLS TEEL VSLG EKMV TAFT TCCT LSEE FACV DNLA DLVF GELC GVNE NRTI NPAV DHCC KTNF AFRR PCFE SLKA DKTY VPPP FSQD LFTF HADM CQSQ NEEL QRKT DRFL VNLV KLKH ELTD EELQ SLFT NFAN VVDK CCKA ESPE VCFN EESP KIGN
Gene ID:173
Uniprot:P43652
Cellular Location:Secreted
Calculated MW:69kDa
Observed MW:69kDa
Synonyms:AFM, ALB2, ALBA, ALF, afamin
Background:This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream.
UniProt Protein Function:A-albumin: Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the regulation and transport of vitamin E at the blood-brain barrier. Belongs to the ALB/AFP/VDB family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Carrier; Secreted

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular region; extracellular space

Molecular Function:vitamin E binding

Biological Process: vitamin transport

NCBI Summary:This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream. [provided by RefSeq, Jul 2008]
UniProt Code:P43652
NCBI GenInfo Identifier:1168366
NCBI Gene ID:173
NCBI Accession:P43652.1
UniProt Secondary Accession:P43652,Q32MR3, Q4W5C5, A8K3E1,
UniProt Related Accession:P43652
Molecular Weight:69,069 Da
NCBI Full Name:Afamin
NCBI Synonym Full Names:afamin
NCBI Official Symbol:AFM  
NCBI Official Synonym Symbols:ALF; ALB2; ALBA  
NCBI Protein Information:afamin
UniProt Protein Name:Afamin
UniProt Synonym Protein Names:Alpha-albumin; Alpha-Alb
Protein Family:Afamin
UniProt Gene Name:AFM  
UniProt Entry Name:AFAM_HUMAN
Anti-AFM Antibody (CAB15253)Western blot analysis of extracts of Rat heart, using AFM antibody (CAB15253) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1S.
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