Cell Biology Antibodies 10

Anti-AFF1 Antibody (CAB6933)

(No reviews yet) Write a Review
SKU:
CAB6933
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-AFF1 Antibody
Antibody SKU:CAB6933
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1124-1218 of human AFF1 (NP_001160165.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:Human, Rat
Positive Samples:SW480, BT474
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1124-1218 of human AFF1 (NP_001160165.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SQSS AGSV GSSG VAAT ISTP VTIQ NMTS SYVT ITSH VLTA FDLW EQAE ALTR KNKE FFAR LSTN VCTL ALNS SLVD LVHY TRQG FQQL QELT KTP
Gene ID:4299
Uniprot:P51825
Cellular Location:Nucleus
Calculated MW:131kDa/132kDa
Observed MW:135kDa
Synonyms:AFF1, AF4, MLLT2, PBM1
Background:This gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:AF-4: A chromosomal aberration involving AFF1 is associated with acute leukemias. Translocation t(4;11)(q21;q23) with MLL/HRX. The result is a rogue activator protein. Belongs to the AF4 family.
UniProt Protein Details:

Protein type:Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: transcription elongation factor complex

Molecular Function:protein binding; transcription factor activity

Biological Process: positive regulation of transcription, DNA-dependent

NCBI Summary:This gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
UniProt Code:P51825
NCBI GenInfo Identifier:1703194
NCBI Gene ID:4299
NCBI Accession:P51825.1
UniProt Secondary Accession:P51825,B4DTU1, E9PBM3,
UniProt Related Accession:P51825
Molecular Weight:1210
NCBI Full Name:AF4/FMR2 family member 1
NCBI Synonym Full Names:AF4/FMR2 family, member 1
NCBI Official Symbol:AFF1  
NCBI Official Synonym Symbols:AF4; PBM1; MLLT2  
NCBI Protein Information:AF4/FMR2 family member 1; protein AF-4; proto-oncogene AF4; pre-B-cell monocytic leukemia partner 1; ALL1-fused gene from chromosome 4 protein; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 2
UniProt Protein Name:AF4/FMR2 family member 1
UniProt Synonym Protein Names:ALL1-fused gene from chromosome 4 protein; Protein AF-4; Protein FEL; Proto-oncogene AF4
Protein Family:AF4/FMR2 family
UniProt Gene Name:AFF1  
UniProt Entry Name:AFF1_HUMAN
Anti-AFF1 Antibody (CAB6933)Western blot analysis of extracts of various cell lines, using AFF1 antibody (CAB6933) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
Anti-AFF1 Antibody (CAB6933)Immunofluorescence analysis of U2OS cells using AFF1 antibody (CAB6933).
View AllClose

0 Reviews

View AllClose

Related Products