Antibody Name:	Anti-AFF1 Antibody
Antibody SKU:	CAB6933
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1124-1218 of human AFF1 (NP_001160165.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Rat
Positive Samples:	SW480, BT474

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1124-1218 of human AFF1 (NP_001160165.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SQSS AGSV GSSG VAAT ISTP VTIQ NMTS SYVT ITSH VLTA FDLW EQAE ALTR KNKE FFAR LSTN VCTL ALNS SLVD LVHY TRQG FQQL QELT KTP
Gene ID:	4299
Uniprot:	P51825
Cellular Location:	Nucleus
Calculated MW:	131kDa/132kDa
Observed MW:	135kDa

Synonyms:

AFF1, AF4, MLLT2, PBM1

Background:

This gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	AF-4: A chromosomal aberration involving AFF1 is associated with acute leukemias. Translocation t(4;11)(q21;q23) with MLL/HRX. The result is a rogue activator protein. Belongs to the AF4 family.
UniProt Protein Details:	Protein type:Oncoprotein; Transcription factor Chromosomal Location of Human Ortholog: 4q21 Cellular Component: transcription elongation factor complex Molecular Function:protein binding; transcription factor activity Biological Process: positive regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
UniProt Code:	P51825
NCBI GenInfo Identifier:	1703194
NCBI Gene ID:	4299
NCBI Accession:	P51825.1
UniProt Secondary Accession:	P51825,B4DTU1, E9PBM3,
UniProt Related Accession:	P51825
Molecular Weight:	1210
NCBI Full Name:	AF4/FMR2 family member 1
NCBI Synonym Full Names:	AF4/FMR2 family, member 1
NCBI Official Symbol:	AFF1
NCBI Official Synonym Symbols:	AF4; PBM1; MLLT2
NCBI Protein Information:	AF4/FMR2 family member 1; protein AF-4; proto-oncogene AF4; pre-B-cell monocytic leukemia partner 1; ALL1-fused gene from chromosome 4 protein; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 2
UniProt Protein Name:	AF4/FMR2 family member 1
UniProt Synonym Protein Names:	ALL1-fused gene from chromosome 4 protein; Protein AF-4; Protein FEL; Proto-oncogene AF4
Protein Family:	AF4/FMR2 family
UniProt Gene Name:	AFF1
UniProt Entry Name:	AFF1_HUMAN

	Western blot analysis of extracts of various cell lines, using AFF1 antibody (CAB6933) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using AFF1 antibody (CAB6933).