Cell Biology Antibodies 8
Anti-AFAP1 Antibody (CAB3478)
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- SKU:
- CAB3478
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-AFAP1 Antibody |
| Antibody SKU: | CAB3478 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 461-730 of human AFAP1 (NP_940997.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:1000 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 22Rv1, A-549, HeLa, U-251MG, HepG2, Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 461-730 of human AFAP1 (NP_940997.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SVIQ TAKQ TFCF MNRR VISA NPYL GGTS NGYA HPSG TALH YDDV PCIN GSLK GKKP PVAS NGVT GKGK TLSS QPKK ADPA AVVK RTGS NAAQ YKYG KNRV EADA KRLQ TKEE ELLK RKEA LRNR LAQL RKER KDLR AAIE VNAG RKPQ AILE EKLK QLEE ECRQ KEAE RVSL ELEL TEVK ESLK KALA GGVT LGLA IEPK SGTS SPQS PVFR HRTL ENSP ISSC DTSD TEGP VPVN SAAV LKKS QAAP GSSP CRGH VLRK AKEW ELKN GT |
| Gene ID: | 60312 |
| Uniprot: | Q8N556 |
| Cellular Location: | Cytoplasm, cytoskeleton |
| Calculated MW: | 80kDa/89kDa |
| Observed MW: | 81kDa |
| Synonyms: | AFAP1, AFAP, AFAP-110, AFAP110 |
| Background: | The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | AFAP: Can cross-link actin filaments into both network and bundle structures. May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 4p16 Cellular Component: focal adhesion; cytoplasm; actin cytoskeleton Molecular Function:actin binding Biological Process: regulation of signal transduction; regulation of cytoskeleton organization and biogenesis |
| NCBI Summary: | The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
| UniProt Code: | Q8N556 |
| NCBI GenInfo Identifier: | 166919564 |
| NCBI Gene ID: | 60312 |
| NCBI Accession: | Q8N556.2 |
| UniProt Secondary Accession: | Q8N556,Q59EY5, Q9HBY1, A8K442, B4DMU2, E9PDT7, |
| UniProt Related Accession: | Q8N556 |
| Molecular Weight: | 730 |
| NCBI Full Name: | Actin filament-associated protein 1 |
| NCBI Synonym Full Names: | actin filament associated protein 1 |
| NCBI Official Symbol: | AFAP1 |
| NCBI Official Synonym Symbols: | AFAP; AFAP110; AFAP-110 |
| NCBI Protein Information: | actin filament-associated protein 1; 110 kDa actin filament-associated protein; actin filament-associated protein, 110 kDa |
| UniProt Protein Name: | Actin filament-associated protein 1 |
| UniProt Synonym Protein Names: | 110 kDa actin filament-associated protein; AFAP-110 |
| Protein Family: | Actin filament-associated protein |
| UniProt Gene Name: | AFAP1 |
| UniProt Entry Name: | AFAP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AFAP1 antibody (CAB3478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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