Antibody Name:	Anti-AES Antibody
Antibody SKU:	CAB7874
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human AES (NP_945320.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	A-549, HeLa, U-251MG, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human AES (NP_945320.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MCHK NGFP QEGG ITAA FLQK RKLR LSKN HRPA RAKV TEHV RGTR PGRA TAGP AAST RAAG SLFF DRWG NRGP AGCR GSSH LPQQ LKFT TSDS CDRI KDEF QLLQ AQYH SLKL ECDK LASE KSEM QRHY VMYY EMSY GLNI EMHK QAEI VKRL NGIC AQVL PYLS QEHQ QQVL GAIE RAKQ VTAP ELNS IIRQ QLQA HQLS QLQA LALP LTPL PVGL QPPS LPAV SAGT GLLS LSAL GSQA HLSK EDKN GHDG DTHQ EDDG EKSD
Gene ID:	166
Uniprot:	Q08117
Cellular Location:	Nucleus
Calculated MW:	21kDa/29kDa
Observed MW:	22kDa

Synonyms:

AES, AES-1, AES-2, ESP1, GRG, GRG5, Grg-5, TLE5

Background:

The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	AES: Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Belongs to the WD repeat Groucho/TLE family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: nucleus Molecular Function:protein binding; transcription corepressor activity Biological Process: organ morphogenesis; Wnt receptor signaling pathway; transcription, DNA-dependent; regulation of growth; multicellular organismal development; cellular response to extracellular stimulus; negative regulation of transcription from RNA polymerase II promoter; negative regulation of protein binding; negative regulation of transcription, DNA-dependent; skeletal development
NCBI Summary:	The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q08117
NCBI GenInfo Identifier:	23503062
NCBI Gene ID:	166
NCBI Accession:	Q08117.4
UniProt Secondary Accession:	Q08117,Q12808, Q14CJ1, Q96TG9, Q9UDY9, B2RBL0,
UniProt Related Accession:	Q08117
Molecular Weight:	29,091 Da
NCBI Full Name:	Amino-terminal enhancer of split
NCBI Synonym Full Names:	amino-terminal enhancer of split
NCBI Official Symbol:	AES
NCBI Official Synonym Symbols:	GRG; ESP1; GRG5; TLE5; AES-1; AES-2
NCBI Protein Information:	amino-terminal enhancer of split; gp130-associated protein GAM
UniProt Protein Name:	Amino-terminal enhancer of split
UniProt Synonym Protein Names:	Gp130-associated protein GAM; Protein ESP1; Protein GRG
Protein Family:	Acetyl esterase
UniProt Gene Name:	AES
UniProt Entry Name:	AES_HUMAN

	Western blot analysis of extracts of various cell lines, using AES antibody (CAB7874) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of A549 cells using AES antibody (CAB7874). Blue: DAPI for nuclear staining.