KO Validated Antibodies 1

Anti-ADRBK1 Antibody (CAB1662)[KO Validated]

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SKU:
CAB1662
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ADRBK1 Antibody [KO Validated]
Antibody SKU:CAB1662
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 440-689 of human ADRBK1 (NP_001610.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, Mouse spleen, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 440-689 of human ADRBK1 (NP_001610.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LGRG AQEV KESP FFRS LDWQ MVFL QKYP PPLI PPRG EVNA ADAF DIGS FDEE DTKG IKLL DSDQ ELYR NFPL TISE RWQQ EVAE TVFD TINA ETDR LEAR KKAK NKQL GHEE DYAL GKDC IMHG YMSK MGNP FLTQ WQRR YFYL FPNR LEWR GEGE APQS LLTM EEIQ SVEE TQIK ERKC LLLK IRGG KQFI LQCD SDPE LVQW KKEL RDAY REAQ QLVQ RVPK MKNK PRSP VVEL SKVP LVQR GSAN GL
Gene ID:156
Uniprot:P25098
Cellular Location:Cell membrane, Cytoplasm
Calculated MW:79kDa
Observed MW:80kDa
Synonyms:GRK2, ADRBK1, BARK1, BETA-ARK1
Background:The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.
UniProt Protein Function:GRK2: a ubiquitous protein kinase of the GRK family. Phosphorylates the beta-2-adrenergic receptor and related G-protein-coupled receptors. Appears to mediate agonist-specific desensitization observed at high agonist concentrations. Expression level is consistently elevated in chronic human heart failure. Mouse models of severe heart failure have been used to demonstrate that inhibition of BARK1 with a peptide inhibitor is sufficient to increase mean survival, reduce dialation and improve cardiac function.
UniProt Protein Details:

Protein type:Kinase, protein; EC 2.7.11.15; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; GRK family; BARK subfamily

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: membrane; cytoplasm; plasma membrane; cytosol

Molecular Function:G-protein coupled receptor kinase activity; protein binding; Edg-2 lysophosphatidic acid receptor binding; beta-adrenergic receptor kinase activity; alpha-2A adrenergic receptor binding; ATP binding; protein kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; desensitization of G-protein coupled receptor protein signaling pathway; tachykinin signaling pathway; fibroblast growth factor receptor signaling pathway; acetylcholine receptor signaling, muscarinic pathway; nerve growth factor receptor signaling pathway; heart development; peptidyl-threonine phosphorylation; signal transduction; negative regulation of striated muscle contraction; peptidyl-serine phosphorylation; negative regulation of the force of heart contraction by chemical signal; phospholipase C activation; receptor internalization; positive regulation of catecholamine secretion; innate immune response; cardiac muscle contraction

NCBI Summary:The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008]
UniProt Code:P25098
NCBI GenInfo Identifier:126302521
NCBI Gene ID:156
NCBI Accession:P25098.2
UniProt Secondary Accession:P25098,Q13837, Q6GTT3, B0ZBE1,
UniProt Related Accession:P25098
Molecular Weight:
NCBI Full Name:Beta-adrenergic receptor kinase 1
NCBI Synonym Full Names:adrenergic, beta, receptor kinase 1
NCBI Official Symbol:ADRBK1  
NCBI Official Synonym Symbols:GRK2; BARK1; BETA-ARK1  
NCBI Protein Information:beta-adrenergic receptor kinase 1; beta-ARK-1; G-protein coupled receptor kinase 2
UniProt Protein Name:Beta-adrenergic receptor kinase 1
UniProt Synonym Protein Names:G-protein coupled receptor kinase 2
UniProt Gene Name:ADRBK1  
UniProt Entry Name:ARBK1_HUMAN
Anti-ADRBK1 Antibody [KO Validated] (CAB1662)Western blot analysis of extracts of various cell lines, using ADRBK1 antibody (CAB1662) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-ADRBK1 Antibody [KO Validated] (CAB1662)Western blot analysis of extracts from normal (control) and ADRBK1 knockout (KO) 293T cells, using ADRBK1 antibody (CAB1662) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-ADRBK1 Antibody [KO Validated] (CAB1662)Immunohistochemistry of paraffin-embedded rat brain using ADRBK1 Antibody (CAB1662) at dilution of 1:200 (40x lens).
Anti-ADRBK1 Antibody [KO Validated] (CAB1662)Immunohistochemistry of paraffin-embedded human esophageal cancer using ADRBK1 Antibody (CAB1662) at dilution of 1:200 (40x lens).
Anti-ADRBK1 Antibody [KO Validated] (CAB1662)Immunohistochemistry of paraffin-embedded mouse kidney using ADRBK1 Antibody (CAB1662) at dilution of 1:200 (40x lens).
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