Name: Anti-ADRB3 Antibody (CAB8607)
Brand: Cell Biology Antibodies 12
SKU: CAB8607
Availability: OutOfStock

Antibody Name:	Anti-ADRB3 Antibody
Antibody SKU:	CAB8607
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ADRB3 (NP_000016.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, K-562

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ADRB3 (NP_000016.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAPW PHEN SSLA PWPD LPTL APNT ANTS GLPG VPWE AALA GALL ALAV LATV GGNL LVIV AIAW TPRL QTMT NVFV TSLA AADL VMGL LVVP PAAT LALT
Gene ID:	155
Uniprot:	P13945
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	43kDa
Observed MW:	47kDa

Synonyms:	ADRB3, BETA3AR
Background:	The protein encoded by this gene belongs to the family of beta adrenergic receptors, which mediate catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor is located mainly in the adipose tissue and is involved in the regulation of lipolysis and thermogenesis.

UniProt Protein Function:	ADRB3: Beta-adrenergic receptors mediate the catecholamine- induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily.
UniProt Protein Details:	Protein type:GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 8p12 Cellular Component: integral to plasma membrane; plasma membrane; receptor complex Molecular Function:beta-adrenergic receptor activity; beta3-adrenergic receptor activity; epinephrine binding; norepinephrine binding; protein binding; protein homodimerization activity Biological Process: adenylate cyclase activation; arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; carbohydrate metabolic process; cell-cell signaling; energy reserve metabolic process; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein signaling, coupled to cyclic nucleotide second messenger; generation of precursor metabolites and energy; norepinephrine-epinephrine vasodilation involved in regulation of systemic arterial blood pressure; positive regulation of MAPKKK cascade; receptor-mediated endocytosis; thermoregulation Disease: Obesity
NCBI Summary:	The protein encoded by this gene belongs to the family of beta adrenergic receptors, which mediate catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor is located mainly in the adipose tissue and is involved in the regulation of lipolysis and thermogenesis. [provided by RefSeq, Feb 2009]
UniProt Code:	P13945
NCBI GenInfo Identifier:	461604
NCBI Gene ID:	155
NCBI Accession:	P13945.3
UniProt Secondary Accession:	P13945,Q4JFT4,
UniProt Related Accession:	P13945
Molecular Weight:	43,519 Da
NCBI Full Name:	Beta-3 adrenergic receptor
NCBI Synonym Full Names:	adrenoceptor beta 3
NCBI Official Symbol:	ADRB3
NCBI Official Synonym Symbols:	BETA3AR
NCBI Protein Information:	beta-3 adrenergic receptor
UniProt Protein Name:	Beta-3 adrenergic receptor
UniProt Synonym Protein Names:	Beta-3 adrenoreceptor; Beta-3 adrenoceptor
Protein Family:	Beta-3 adrenergic receptor
UniProt Gene Name:	ADRB3
UniProt Entry Name:	ADRB3_HUMAN

	Western blot analysis of extracts of various cell lines, using ADRB3 antibody (CAB8607) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of NIH/3T3 cells using ADRB3 Rabbit pAb (CAB8607) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using ADRB3 Rabbit pAb (CAB8607) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of C6 cells using ADRB3 Rabbit pAb (CAB8607) at dilution of 1:100. Blue: DAPI for nuclear staining.