Cell Biology Antibodies 5
Anti-ADRA1A Antibody (CAB1471)
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- SKU:
- CAB1471
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ADRA1A Antibody |
| Antibody SKU: | CAB1471 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 346-475 of human ADRA1A (NP_150646.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, LO2, HL-60, Mouse heart, Mouse kidney, Mouse spinal cord, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 346-475 of human ADRA1A (NP_150646.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LCRK QSSK HALG YTLH PPSQ AVEG QHKD MVRI PVGS RETF YRIS KTDG VCEW KFFS SMPR GSAR ITVS KDQS SCTT ARTK SRSV TRLE CSGM ILAH CNLR LPGS RDSP ASAS QAAG TTGM CHQA DATR PS |
| Gene ID: | 148 |
| Uniprot: | P35348 |
| Cellular Location: | Cell membrane, Multi-pass membrane protein, Nucleus membrane |
| Calculated MW: | 32-52kDa |
| Observed MW: | 51kDa |
| Synonyms: | ADRA1A, ADRA1C, ADRA1L1, ALPHA1AAR |
| Background: | Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. |
| UniProt Protein Function: | This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes. |
| NCBI Summary: | Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P35348 |
| NCBI GenInfo Identifier: | 1168246 |
| NCBI Gene ID: | 148 |
| NCBI Accession: | P35348.2 |
| UniProt Secondary Accession: | P35348,O60451, Q13675, Q13729, A8K0I3, B0ZBD1, B0ZBD2 B0ZBD4, B0ZBD5, B0ZBD6, B0ZBD8, B0ZBD9, |
| UniProt Related Accession: | P35348 |
| Molecular Weight: | 40,638 Da |
| NCBI Full Name: | Alpha-1A adrenergic receptor |
| NCBI Synonym Full Names: | adrenoceptor alpha 1A |
| NCBI Official Symbol: | ADRA1A |
| NCBI Official Synonym Symbols: | ADRA1C; ADRA1L1; ALPHA1AAR |
| NCBI Protein Information: | alpha-1A adrenergic receptor |
| UniProt Protein Name: | Alpha-1A adrenergic receptor |
| UniProt Synonym Protein Names: | Alpha-1A adrenoreceptor; Alpha-1A adrenoceptor; Alpha-1C adrenergic receptor; Alpha-adrenergic receptor 1c |
| Protein Family: | Alpha-1A adrenergic receptor |
| UniProt Gene Name: | ADRA1A |
| UniProt Entry Name: | ADA1A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ADRA1A antibody (CAB1471) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat lung using ADRA1A antibody (CAB1471) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat heart using ADRA1A antibody (CAB1471) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using ADRA1A antibody (CAB1471) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using ADRA1A antibody (CAB1471) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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