Cell Biology Antibodies 10

Anti-ADPRH Antibody (CAB6277)

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SKU:
CAB6277
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-ADPRH Antibody
Antibody SKU:CAB6277
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 180-270 of human ADPRH (NP_001116.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:K-562, SW480, Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 180-270 of human ADPRH (NP_001116.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AYAV NSRP PLQW GKGL MELL PEAK KYIV QSGY FVEE NLQH WSYF QTKW ENYL KLRG ILDG ESAP TFPE SFGV KERD QFYT SLSY SGWG GSS
Gene ID:141
Uniprot:P54922
Cellular Location:
Calculated MW:39kDa
Observed MW:39kDa
Synonyms:ADPRH, ARH1
Background:The enzyme encoded by this gene catalyzes removal of mono-ADP-ribose from arginine residues of proteins in the ADP-ribosylation cycle. Unlike the rat and mouse enzymes that require DTT for maximal activity, the human enzyme is DTT-independent. Alternatively spliced transcript variants that encode different protein isoforms have been described.
UniProt Protein Function:ADPRH: Catalyzes the reverse reaction of mono-ADP-ribosylation. Belongs to the ADP-ribosylglycohydrolase family.
UniProt Protein Details:

Protein type:Hydrolase; EC 3.2.2.19

Chromosomal Location of Human Ortholog: 3q13.31-q13.33

Molecular Function:ADP-ribosylarginine hydrolase activity; magnesium ion binding

Biological Process: protein amino acid de-ADP-ribosylation; protein modification process

NCBI Summary:The enzyme encoded by this gene catalyzes removal of mono-ADP-ribose from arginine residues of proteins in the ADP-ribosylation cycle. Unlike the rat and mouse enzymes that require DTT for maximal activity, the human enzyme is DTT-independent. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, May 2014]
UniProt Code:P54922
NCBI GenInfo Identifier:1703392
NCBI Gene ID:141
NCBI Accession:P54922.1
UniProt Secondary Accession:P54922,B2R8H1, D3DN83,
UniProt Related Accession:P54922
Molecular Weight:357
NCBI Full Name:[Protein ADP-ribosylarginine] hydrolase
NCBI Synonym Full Names:ADP-ribosylarginine hydrolase
NCBI Official Symbol:ADPRH  
NCBI Official Synonym Symbols:ARH1  
NCBI Protein Information:ADP-ribosylarginine hydrolase; ADP-ribose-L-arginine cleaving enzyme
UniProt Protein Name:[Protein ADP-ribosylarginine] hydrolase
UniProt Synonym Protein Names:ADP-ribose-L-arginine cleaving enzyme
Protein Family:[Protein ADP-ribosylarginine] hydrolase
UniProt Gene Name:ADPRH  
UniProt Entry Name:ADPRH_HUMAN
Anti-ADPRH Antibody (CAB6277)Western blot analysis of extracts of various cell lines, using ADPRH antibody (CAB6277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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system_update_altDatasheetHuman ADPRH/ARH1 Recombinant Protein ADP-Ribosylarginine Hydrolase (ADPRH) belongs to the ADP-Ribosylglycohydrolase family. ADPRH catalyzes removal of mono-ADP-ribose fr