Metabolism Antibodies 2
Anti-ADPGK Antibody (CAB7172)
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- SKU:
- CAB7172
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-ADPGK Antibody |
| Antibody SKU: | CAB7172 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 217-496 of human ADPGK (NP_112574.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HL-60, THP-1, HepG2, Jurkat, HeLa, Mouse spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 217-496 of human ADPGK (NP_112574.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EWGQ LKAP HANR FIFS HDLS NGAM NMLE VFVS SLEE FQPD LVVL SGLH MMEG QSKE LQRK RLLE VVTS ISDI PTGI PVHL ELAS MTNR ELMS SIVH QVFP AVTS LGLN EQEL LFLT QSAS GPHS SLSS WNGV PDVG MVSD ILFW ILKE HGRS KSRA SDLT RIHF HTLV YHIL ATVD GHWA NQLA AVAA GARV AGTQ ACAT ETID TSRV SLRA PQEF MTSH SEAG SRIV LNPN KPVV EWHR EGIS FHFT PVLV CKDP IRTV GLGD AISA EGLF YSEV HPHY |
| Gene ID: | 83440 |
| Uniprot: | Q9BRR6 |
| Cellular Location: | Secreted |
| Calculated MW: | 8kDa/24kDa/28kDa/38kDa/53kDa/54kDa |
| Observed MW: | 54kDa |
| Synonyms: | ADPGK, 2610017G09Rik, ADP-GK |
| Background: |
| UniProt Protein Function: | ADPGK: Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency. Belongs to the ADP-dependent glucokinase family. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.7.1.147; Secreted, signal peptide; Kinase, other; Secreted Chromosomal Location of Human Ortholog: 15q24.1 Cellular Component: membrane; extracellular region Molecular Function:ADP-specific glucokinase activity; metal ion binding Biological Process: glycolysis; phosphorylation |
| NCBI Summary: | ADPGK (EC 2.7.1.147) catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions (Ronimus and Morgan, 2004 [PubMed 14975750]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9BRR6 |
| NCBI GenInfo Identifier: | 62899887 |
| NCBI Gene ID: | 83440 |
| NCBI Accession: | Q9BRR6.1 |
| UniProt Secondary Accession: | Q9BRR6,Q49AU7, Q8NBI1, Q8WZ90, Q96NF8, Q9H0A7, |
| UniProt Related Accession: | Q9BRR6 |
| Molecular Weight: | 8,252 Da |
| NCBI Full Name: | ADP-dependent glucokinase |
| NCBI Synonym Full Names: | ADP-dependent glucokinase |
| NCBI Official Symbol: | ADPGK |
| NCBI Official Synonym Symbols: | ADP-GK; 2610017G09Rik |
| NCBI Protein Information: | ADP-dependent glucokinase; rbBP-35; ATP-dependent glucokinase |
| UniProt Protein Name: | ADP-dependent glucokinase |
| UniProt Synonym Protein Names: | RbBP-35 |
| Protein Family: | ADP-dependent glucokinase |
| UniProt Gene Name: | ADPGK |
| UniProt Entry Name: | ADPGK_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ADPGK antibody (CAB7172) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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