KO Validated Antibodies 2

Anti-ADK Antibody (CAB19992)[KO Validated]

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SKU:
CAB19992
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-ADK Antibody [KO Validated]
Antibody SKU:CAB19992
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human ADK.
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant protein of human ADK.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:132
Uniprot:P55263
Cellular Location:Cytoplasm, Nucleus
Calculated MW:34kDa/36kDa/38kDa/40kDa
Observed MW:45KDa
Anti-ADK Antibody [KO Validated] (CAB19992)Western blot analysis of extracts from normal (control) and ADK knockout (KO) HeLa cells, using ADK antibody (CAB19992) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Synonyms:ADK, AK
Background:This gene an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:ADK: ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides. Defects in ADK are the cause of hypermethioninemia due to adenosine kinase deficiency (HMAKD). A metabolic disorder characterized by global developmental delay, early-onset seizures, mild dysmorphic features, and characteristic biochemical anomalies, including persistent hypermethioninemia with increased levels of S-adenosylmethionine and S-adenosylhomocysteine. Homocysteine levels are typically normal. Belongs to the carbohydrate kinase PfkB family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Kinase, other; Nucleotide Metabolism - purine; EC 2.7.1.20

Chromosomal Location of Human Ortholog: 10q22|10q11-q24

Cellular Component: cytoplasm; cytosol; nucleoplasm

Molecular Function:adenosine kinase activity

Biological Process: purine salvage; ribonucleoside monophosphate biosynthetic process

Disease: Hypermethioninemia Due To Adenosine Kinase Deficiency

NCBI Summary:This gene an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
UniProt Code:P55263
NCBI GenInfo Identifier:6840802
NCBI Gene ID:132
NCBI Accession:P55263.2
UniProt Secondary Accession:P55263,O00741, O00742, Q16710, Q5JQ10, Q5JQ11, Q9BTN2 B7Z783, B7Z800,
UniProt Related Accession:P55263
Molecular Weight:36,580 Da
NCBI Full Name:Adenosine kinase
NCBI Synonym Full Names:adenosine kinase
NCBI Official Symbol:ADK  
NCBI Official Synonym Symbols:AK  
NCBI Protein Information:adenosine kinase
UniProt Protein Name:Adenosine kinase
UniProt Synonym Protein Names:Adenosine 5'-phosphotransferase
UniProt Gene Name:ADK  
UniProt Entry Name:ADK_HUMAN
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