Antibody Name:	Anti-ADH4 Antibody
Antibody SKU:	CAB5454
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-380 of human ADH4 (NP_000661.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-380 of human ADH4 (NP_000661.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGTK GKVI KCKA AIAW EAGK PLCI EEVE VAPP KAHE VRIQ IIAT SLCH TDAT VIDS KFEG LAFP VIVG HEAA GIVE SIGP GVTN VKPG DKVI PLYA PLCR KCKF CLSP LTNL CGKI SNLK SPAS DQQL MEDK TSRF TCKG KPVY HFFG TSTF SQYT VVSD INLA KIDD DANL ERVC LLGC GFST GYGA AINN AKVT PGST CAVF GLGG VGLS AVMG CKAA GASR IIGI DINS EKFV KAKA LGAT DCLN PRDL HKPI QEVI IELT KGGV DFAL DCAG GSET MKAA LDCT TAGW GSCT FIGV AAGS KGLT IFPE ELII GRTI NGTF FGGW KSVD SIPK LVTD YKNK KFNL DALV THTL PFDK ISEA FDLM NQGK SVRT ILIF
Gene ID:	127
Uniprot:	P08319
Cellular Location:	Cytoplasm
Calculated MW:	40kDa/42kDa
Observed MW:	45kDa

Synonyms:

ADH4, ADH-2, HEL-S-4

Background:

This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes.

UniProt Protein Function:	ADH4: class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.1.1.1; Xenobiotic Metabolism - metabolism by cytochrome P450; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Amino Acid Metabolism - tyrosine; Oxidoreductase; Lipid Metabolism - fatty acid Chromosomal Location of Human Ortholog: 4q22 Cellular Component: cytosol Molecular Function:zinc ion binding; NADPH:quinone reductase activity; alcohol dehydrogenase activity; all-trans retinal binding; alcohol dehydrogenase activity, zinc-dependent; ethanol binding; aldehyde reductase activity; benzaldehyde dehydrogenase activity; NAD binding; retinol binding; retinol dehydrogenase activity; oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor Biological Process: retinol metabolic process; xenobiotic metabolic process; alcohol metabolic process; alcohol catabolic process; aldehyde metabolic process; ethanol oxidation; retinoid metabolic process
NCBI Summary:	This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes. [provided by RefSeq, Jul 2008]
UniProt Code:	P08319
NCBI GenInfo Identifier:	308153684
NCBI Gene ID:	127
NCBI Accession:	P08319.5
UniProt Secondary Accession:	P08319,Q8TCD7, A8K470, B4DIE7, C9J4A9,
UniProt Related Accession:	P08319
Molecular Weight:	380
NCBI Full Name:	Alcohol dehydrogenase 4
NCBI Synonym Full Names:	alcohol dehydrogenase 4 (class II), pi polypeptide
NCBI Official Symbol:	ADH4
NCBI Official Synonym Symbols:	ADH-2; HEL-S-4
NCBI Protein Information:	alcohol dehydrogenase 4; aldehyde reductase; epididymis secretory protein Li 4; alcohol dehydrogenase class II pi chain
UniProt Protein Name:	Alcohol dehydrogenase 4
UniProt Synonym Protein Names:	Alcohol dehydrogenase class II pi chain
Protein Family:	Alcohol dehydrogenase
UniProt Gene Name:	ADH4
UniProt Entry Name:	ADH4_HUMAN

	Western blot analysis of extracts of mouse liver, using ADH4 antibody (CAB5454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat kidney using ADH4 antibody (CAB5454) at dilution of 1:200 (40x lens).