Cell Biology Antibodies 6
Anti-ADD1 Antibody (CAB1592)
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- SKU:
- CAB1592
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ADD1 Antibody |
| Antibody SKU: | CAB1592 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 60-300 of human ADD1 (NP_054908.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | BT-474, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 60-300 of human ADD1 (NP_054908.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MILQ SPAF CEEL ESMI QEQF KKGK NPTG LLAL QQIA DFMT TNVP NVYP AAPQ GGMA ALNM SLGM VTPV NDLR GSDS IAYD KGEK LLRC KLAA FYRL ADLF GWSQ LIYN HITT RVNS EQEH FLIV PFGL LYSE VTAS SLVK INLQ GDIV DRGS TNLG VNQA GFTL HSAI YAAR PDVK CVVH IHTP AGAA VSAM KCGL LPIS PEAL SLGE VAYH DYHG ILVD EEEK VLIQ KNLG PKSK VLIL R |
| Gene ID: | 118 |
| Uniprot: | P35611 |
| Cellular Location: | Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cytoskeleton |
| Calculated MW: | 56kDa/69kDa/73kDa/80kDa/84kDa |
| Observed MW: | 73kDa |
| Synonyms: | ADD1, ADDA, adducin 1 |
| Background: | Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. |
| UniProt Protein Function: | Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. |
| NCBI Summary: | Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, and gamma). Adducin acts as a heterodimer of the related alpha, beta, or gamma subunits. The protein encoded by this gene represents the alpha subunit. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. [provided by RefSeq, Aug 2017] |
| UniProt Code: | P35611 |
| NCBI GenInfo Identifier: | 12644231 |
| NCBI Gene ID: | 118 |
| NCBI Accession: | P35611.2 |
| UniProt Secondary Accession: | P35611,Q13734, Q14729, Q16156, Q86XM2, Q9UJB6, A2A3N8 A2A3P0, B4DI79, D3DVR3, D3DVR4, D3DVR5, |
| UniProt Related Accession: | P35611 |
| Molecular Weight: | 73,333 Da |
| NCBI Full Name: | Alpha-adducin |
| NCBI Synonym Full Names: | adducin 1 |
| NCBI Official Symbol: | ADD1 |
| NCBI Official Synonym Symbols: | ADDA |
| NCBI Protein Information: | alpha-adducin |
| UniProt Protein Name: | Alpha-adducin |
| UniProt Synonym Protein Names: | Erythrocyte adducin subunit alpha |
| Protein Family: | Alpha-adducin |
| UniProt Gene Name: | ADD1 |
 | Western blot analysis of extracts of various cell lines, using ADD1 antibody (CAB1592) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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