Immunology Antibodies 2

Anti-ADARB1 Antibody (CAB16748)

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SKU:
CAB16748
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-ADARB1 Antibody
Antibody SKU:CAB16748
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 180-380 of human ADARB1 (NP_001103.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-251MG, HeLa, Jurkat, HepG2, mouse brain, mouse lung, mouse testis, rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 180-380 of human ADARB1 (NP_001103.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FETP DKAE PPFY VGSN GDDS FSSS GDLS LSAS PVPA SLAQ PPLP VLPP FPPP SGKN PVMI LNEL RPGL KYDF LSES GESH AKSF VMSV VVDG QFFE GSGR NKKL AKAR AAQS ALAA IFNL HLDQ TPSR QPIP SEGL QLHL PQVL ADAV SRLV LGKF GDLT DNFS SPHA RRKV LAGV VMTT GTDV KDAK VISV STGT KCIN G
Gene ID:104
Uniprot:P78563
Cellular Location:
Calculated MW:80kDa
Observed MW:81kDa
Synonyms:ADARB1, ADAR2, DRABA2, DRADA2, RED1
Background:This gene encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region.
UniProt Protein Function:Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2 and GRIK2) and serotonin (HTR2C), GABA receptor (GABRA3) and potassium voltage-gated channel (KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. Can exert a proviral effect towards human immunodeficiency virus type 1 (HIV-1) and enhances its replication via both an editing-dependent and editing-independent mechanism. The former involves editing of adenosines in the 5'UTR while the latter occurs via suppression of EIF2AK2/PKR activation and function. Can inhibit cell proliferation and migration and can stimulate exocytosis.
NCBI Summary:This gene encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region. [provided by RefSeq, Jul 2008]
UniProt Code:P78563
NCBI GenInfo Identifier:4501919
NCBI Gene ID:104
NCBI Accession:NP_001103.1
UniProt Secondary Accession:P78563,O00395, O00465, O00691, O00692, P78555, A6NFK8 A6NJ84, C3TTQ1, C3TTQ2, C9JUP4, G5E9B4,
UniProt Related Accession:P78563
Molecular Weight:73,665 Da
NCBI Full Name:double-stranded RNA-specific editase 1 isoform 1
NCBI Synonym Full Names:adenosine deaminase, RNA specific B1
NCBI Official Symbol:ADARB1  
NCBI Official Synonym Symbols:RED1; ADAR2; DRABA2; DRADA2  
NCBI Protein Information:double-stranded RNA-specific editase 1
UniProt Protein Name:Double-stranded RNA-specific editase 1
UniProt Synonym Protein Names:RNA-editing deaminase 1; RNA-editing enzyme 1; dsRNA adenosine deaminase
UniProt Gene Name:ADARB1  
Anti-ADARB1 Antibody (CAB16748)Western blot analysis of extracts of various cell lines, using ADARB1 antibody (CAB16748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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