Immunology Antibodies 2

Anti-ADAR Antibody (CAB7869)

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SKU:
CAB7869
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-ADAR Antibody
Antibody SKU:CAB7869
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human ADAR (NP_001102.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, U-87MG, Mouse brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human ADAR (NP_001102.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LGEG KATT AHDL SGKL GTPK KEIN RVLY SLAK KGKL QKEA GTPP LWKI AVST QAWN QHSG VVRP DGHS QGAP NSDP SLEP EDRN STSV SEDL LEPF IAVS A
Gene ID:103
Uniprot:P55265
Cellular Location:Cytoplasm, Nucleus, Nucleus, nucleolus
Calculated MW:103kDa/131kDa/133kDa/136kDa/140kDa
Observed MW:120KDa/150KDa
Synonyms:ADAR, ADAR1, AGS6, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88DSRBP, P136
Background:This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:ADAR: Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site- selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression. Isoform 1 is induced by interferon alpha. Isoform 5 is constitutively expressed. Homodimer. Isoform 1 interacts with ILF2/NF45 and ILF3/NF90. 5 isoforms of the human protein are produced by alternative promoter.
UniProt Protein Details:

Protein type:EC 3.5.4.37; Hydrolase; Nucleolus; RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nucleus

Molecular Function:double-stranded RNA adenosine deaminase activity; protein binding; DNA binding; metal ion binding

Biological Process: positive regulation of viral genome replication; base conversion or substitution editing; in utero embryonic development; response to virus; cytokine and chemokine mediated signaling pathway; miRNA-mediated gene silencing, miRNA loading onto RISC; somatic diversification of immune receptors via somatic mutation; adenosine to inosine editing; osteoblast differentiation; negative regulation of viral genome replication; pre-microRNA processing; protein import into nucleus; mRNA modification; erythrocyte differentiation; innate immune response; hemopoietic progenitor cell differentiation; gene expression; protein export from nucleus; mRNA processing; defense response to virus; negative regulation of apoptosis

Disease: Dyschromatosis Symmetrica Hereditaria; Aicardi-goutieres Syndrome 6

NCBI Summary:This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]
UniProt Code:P55265
NCBI GenInfo Identifier:70167113
NCBI Gene ID:103
NCBI Accession:NP_001020278.1
UniProt Secondary Accession:P55265,O15223, O43859, O43860, Q9BYM3, Q9BYM4, B1AQQ9 B1AQR0, D3DV76,
UniProt Related Accession:P55265
Molecular Weight:
NCBI Full Name:double-stranded RNA-specific adenosine deaminase isoform d
NCBI Synonym Full Names:adenosine deaminase, RNA-specific
NCBI Official Symbol:ADAR  
NCBI Official Synonym Symbols:DSH; AGS6; G1P1; IFI4; P136; ADAR1; DRADA; DSRAD; IFI-4; K88DSRBP  
NCBI Protein Information:double-stranded RNA-specific adenosine deaminase
UniProt Protein Name:Double-stranded RNA-specific adenosine deaminase
UniProt Synonym Protein Names:136 kDa double-stranded RNA-binding protein; p136; Interferon-inducible protein 4; IFI-4; K88DSRBP
Protein Family:Double-stranded RNA-specific editase
UniProt Gene Name:ADAR  
UniProt Entry Name:DSRAD_HUMAN
Anti-ADAR Antibody (CAB7869)Western blot analysis of extracts of various cell lines, using ADAR Rabbit pAb (CAB7869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-ADAR Antibody (CAB7869)Immunohistochemistry of paraffin-embedded rat spleen using ADAR antibody (CAB7869) at dilution of 1:100 (40x lens).
Anti-ADAR Antibody (CAB7869)Immunohistochemistry of paraffin-embedded human colon carcinoma using ADAR antibody (CAB7869) at dilution of 1:100 (40x lens).
Anti-ADAR Antibody (CAB7869)Immunohistochemistry of paraffin-embedded mouse spleen using ADAR antibody (CAB7869) at dilution of 1:100 (40x lens).
Anti-ADAR Antibody (CAB7869)Immunofluorescence analysis of HeLa cells using ADAR antibody (CAB7869) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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