Cell Biology Antibodies 8
Anti-ADAMTS4 Antibody (CAB2525)
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- SKU:
- CAB2525
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ADAMTS4 Antibody |
| Antibody SKU: | CAB2525 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 52-315 of human ADAMTS4 (NP_005090.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, A-549, SKOV3, 293T, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 52-315 of human ADAMTS4 (NP_005090.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ASPL PREE EIVF PEKL NGSV LPGS GAPA RLLC RLQA FGET LLLE LEQD SGVQ VEGL TVQY LGQA PELL GGAE PGTY LTGT INGD PESV ASLH WDGG ALLG VLQY RGAE LHLQ PLEG GTPN SAGG PGAH ILRR KSPA SGQG PMCN VKAP LGSP SPRP RRAK RFAS LSRF VETL VVAD DKMA AFHG AGLK RYLL TVMA AAAK AFKH PSIR NPVS LVVT RLVI LGSG EEGP QVGP SAAQ TLRS FCAW QRGL NTPE DSDP DHFD TAIL |
| Gene ID: | 9507 |
| Uniprot: | O75173 |
| Cellular Location: | Secreted, extracellular matrix, extracellular space |
| Calculated MW: | 90kDa |
| Observed MW: | 80kDa |
| Synonyms: | ADAMTS4, ADAMTS-2, ADAMTS-4, ADMP-1 |
| Background: | This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of this family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The expression of this gene is upregulated in arthritic disease and this may contribute to disease progression through the degradation of aggrecan. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. |
| UniProt Protein Function: | ADAMTS4: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-|-Ala-393' site. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Secreted, signal peptide; Protease; Secreted; EC 3.4.24.82 Chromosomal Location of Human Ortholog: 1q21-q23 Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region Molecular Function:peptidase activity; protein binding; protease binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; defense response to bacterium; proteolysis; skeletal development |
| NCBI Summary: | This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O75173 |
| NCBI GenInfo Identifier: | 317373445 |
| NCBI Gene ID: | 9507 |
| NCBI Accession: | O75173.3 |
| UniProt Secondary Accession: | O75173,Q5VTW2, Q6P4Q8, Q6UWA8, Q9UN83, |
| UniProt Related Accession: | O75173 |
| Molecular Weight: | 90,197 Da |
| NCBI Full Name: | A disintegrin and metalloproteinase with thrombospondin motifs 4 |
| NCBI Synonym Full Names: | ADAM metallopeptidase with thrombospondin type 1 motif, 4 |
| NCBI Official Symbol: | ADAMTS4 |
| NCBI Official Synonym Symbols: | ADMP-1; ADAMTS-2; ADAMTS-4 |
| NCBI Protein Information: | A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAM-TS4; ADAM-TS 4; aggrecanase-1; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4 |
| UniProt Protein Name: | A disintegrin and metalloproteinase with thrombospondin motifs 4 |
| UniProt Synonym Protein Names: | ADMP-1; Aggrecanase-1 |
| Protein Family: | A disintegrin and metalloproteinase with thrombospondin motifs |
| UniProt Gene Name: | ADAMTS4 |
| UniProt Entry Name: | ATS4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ADAMTS4 antibody (CAB2525) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of C6 cells using ADAMTS4 Polyclonal Antibody (CAB2525) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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