Antibody Name:	Anti-ADAMTS13 Antibody
Antibody SKU:	CAB8482
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1300-1400 of human ADAMTS13 (NP_620594.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, LO2, 22Rv1, U-251MG, Mouse liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1300-1400 of human ADAMTS13 (NP_620594.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DMQL FGPW GEIV SPSL SPAT SNAG GCRL FINV APHA RIAI HALA TNMG AGTE GANA SYIL IRDT HSLR TTAF HGQQ VLYW ESES SQAE MEFS EGFL KAQA S
Gene ID:	11093
Uniprot:	Q76LX8
Cellular Location:	Secreted
Calculated MW:	39kDa/144kDa/147kDa/153kDa
Observed MW:	154kDa

Synonyms:

ADAMTS13, ADAM-TS13, ADAMTS-13, C9orf8, VWFCP, vWF-CP

Background:

This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	ADAMTS13: Cleaves the vWF multimers in plasma into smaller forms. Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP); also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Calcium-binding; Secreted, signal peptide; Secreted; Protease; EC 3.4.24.87; Extracellular matrix Chromosomal Location of Human Ortholog: 9q34 Cellular Component: endoplasmic reticulum lumen; proteinaceous extracellular matrix Molecular Function:calcium ion binding; integrin binding; metallopeptidase activity; protein binding; zinc ion binding Biological Process: peptide catabolic process; protein processing; proteolysis Disease: Thrombotic Thrombocytopenic Purpura, Congenital
NCBI Summary:	This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Code:	Q76LX8
NCBI GenInfo Identifier:	74749836
NCBI Gene ID:	11093
NCBI Accession:	Q76LX8.1
UniProt Secondary Accession:	Q76LX8,Q6UY16, Q710F6, Q711T8, Q96L37, Q9H0G3, Q9UGQ1
UniProt Related Accession:	Q76LX8
Molecular Weight:	39,864 Da
NCBI Full Name:	A disintegrin and metalloproteinase with thrombospondin motifs 13
NCBI Synonym Full Names:	ADAM metallopeptidase with thrombospondin type 1 motif 13
NCBI Official Symbol:	ADAMTS13
NCBI Official Synonym Symbols:	VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13
NCBI Protein Information:	A disintegrin and metalloproteinase with thrombospondin motifs 13
UniProt Protein Name:	A disintegrin and metalloproteinase with thrombospondin motifs 13
UniProt Synonym Protein Names:	von Willebrand factor-cleaving protease; vWF-CP; vWF-cleaving protease
Protein Family:	A disintegrin and metalloproteinase with thrombospondin motifs
UniProt Gene Name:	ADAMTS13
UniProt Entry Name:	ATS13_HUMAN

	Western blot analysis of extracts of various cell lines, using ADAMTS13 antibody (CAB8482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of NIH-3T3 cells using Anti-ADAMTS13 Polyclonal Antibody (CAB8482) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.