Antibody Name:	Anti-ADAM9 Antibody
Antibody SKU:	CAB5388
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 475-685 of human ADAM9 (NP_003807.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse kidney, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 475-685 of human ADAM9 (NP_003807.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GKTS ECDV PEYC NGSS QFCQ PDVF IQNG YPCQ NNKA YCYN GMCQ YYDA QCQV IFGS KAKA APKD CFIE VNSK GDRF GNCG FSGN EYKK CATG NALC GKLQ CENV QEIP VFGI VPAI IQTP SRGT KCWG VDFQ LGSD VPDP GMVN EGTK CGAG KICR NFQC VDAS VLNY DCDV QKKC HGHG VCNS NKNC HCEN GWAP PNCE TKGY GGSV DSG
Gene ID:	8754
Uniprot:	Q13443
Cellular Location:	Cell membrane, Secreted, Single-pass type I membrane protein
Calculated MW:	72kDa/90kDa
Observed MW:	100kDa

Synonyms:

ADAM9, CORD9, MCMP, MDC9, Mltng

Background:

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene.

UniProt Protein Function:	ADAM9: Probable zinc protease. May mediate cell-cell or cell- matrix interactions. Isoform 2 displays alpha-secretase activity for APP. Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protease; EC 3.4.24.-; Membrane protein, integral; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 8p11.22 Cellular Component: extracellular space; focal adhesion; cell surface; basolateral plasma membrane; cytoplasm; integral to membrane Molecular Function:integrin binding; collagen binding; protein binding; protein kinase C binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; laminin binding; SH3 domain binding Biological Process: integrin-mediated signaling pathway; positive regulation of keratinocyte migration; extracellular matrix organization and biogenesis; activation of MAPKK activity; cell-matrix adhesion; response to glucocorticoid stimulus; membrane protein ectodomain proteolysis; positive regulation of cell adhesion mediated by integrin; positive regulation of membrane protein ectodomain proteolysis; response to manganese ion; keratinocyte differentiation; collagen catabolic process; extracellular matrix disassembly; PMA-inducible membrane protein ectodomain proteolysis; monocyte activation; response to hydrogen peroxide; positive regulation of protein secretion; transforming growth factor beta receptor signaling pathway; cell-cell adhesion mediated by integrin; cell adhesion mediated by integrin; response to calcium ion; cell adhesion Disease: Cone-rod Dystrophy 9
NCBI Summary:	This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2010]
UniProt Code:	Q13443
NCBI GenInfo Identifier:	24211441
NCBI Gene ID:	8754
NCBI Accession:	Q13443.1
UniProt Secondary Accession:	Q13443,Q10718, Q8NFM6, B7ZLN7,
UniProt Related Accession:	Q13443
Molecular Weight:	72,359 Da
NCBI Full Name:	Disintegrin and metalloproteinase domain-containing protein 9
NCBI Synonym Full Names:	ADAM metallopeptidase domain 9
NCBI Official Symbol:	ADAM9
NCBI Official Synonym Symbols:	MCMP; MDC9; CORD9; Mltng
NCBI Protein Information:	disintegrin and metalloproteinase domain-containing protein 9; cone rod dystrophy 9; myeloma cell metalloproteinase; cellular disintegrin-related protein; ADAM metallopeptidase domain 9 (meltrin gamma); metalloprotease/disintegrin/cysteine-rich protein 9
UniProt Protein Name:	Disintegrin and metalloproteinase domain-containing protein 9
UniProt Synonym Protein Names:	Cellular disintegrin-related protein; Meltrin-gamma; Metalloprotease/disintegrin/cysteine-rich protein 9; Myeloma cell metalloproteinase
Protein Family:	Disintegrin and metalloproteinase domain-containing protein
UniProt Gene Name:	ADAM9
UniProt Entry Name:	ADAM9_HUMAN

	Western blot analysis of extracts of various cell lines, using ADAM9 antibody (CAB5388) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human stomach using ADAM9 antibody (CAB5388) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using ADAM9 Rabbit pAb (CAB5388) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using ADAM9 Rabbit pAb (CAB5388) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of C6 cells using ADAM9 Rabbit pAb (CAB5388) at dilution of 1:100. Blue: DAPI for nuclear staining.