Cell Biology Antibodies 5

Anti-ADAM28 Antibody (CAB14849)

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SKU:
CAB14849
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ADAM28 Antibody
Antibody SKU:CAB14849
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 199-440 of human ADAM28 (NP_055080.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:SGC-7901, HT-29, THP-1, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 199-440 of human ADAM28 (NP_055080.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VQEH EKYI EYYL VLDN GEFK RYNE NQDE IRKR VFEM ANYV NMLY KKLN THVA LVGM EIWT DKDK IKIT PNAS FTLE NFSK WRGS VLSR RKRH DIAQ LITA TELA GTTV GLAF MSTM CSPY SVGV VQDH SDNL LRVA GTMA HEMG HNFG MFHD DYSC KCPS TICV MDKA LSFY IPTD FSSC SRLS YDKF FEDK LSNC LFNA PLPT DIIS TPIC GNQL VEMG EDCD CGTS EECT NICC DAKT CK
Gene ID:10863
Uniprot:Q9UKQ2
Cellular Location:Cell membrane, Secreted, Single-pass type I membrane protein
Calculated MW:61kDa/87kDa
Observed MW:87kDa
Synonyms:ADAM28, ADAM 28, MDC-L, MDCL, eMDC II, eMDCII
Background:This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein. This gene is present in a gene cluster with other members of the ADAM family on chromosome 8. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:ADAM28: May play a role in the adhesive and proteolytic events that occur during lymphocyte emigration or may function in ectodomain shedding of lymphocyte surface target proteins, such as FASL and CD40L. May be involved in sperm maturation. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.4.24.-; Membrane protein, integral; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 8p21.2

Molecular Function:metallopeptidase activity

Biological Process: spermatogenesis

NCBI Summary:This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein. This gene is present in a gene cluster with other members of the ADAM family on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
UniProt Code:Q9UKQ2
NCBI GenInfo Identifier:748983380
NCBI Gene ID:10863
NCBI Accession:NP_001291280.1
UniProt Related Accession:Q9UKQ2
Molecular Weight:
NCBI Full Name:disintegrin and metalloproteinase domain-containing protein 28 isoform 4 preproprotein
NCBI Synonym Full Names:ADAM metallopeptidase domain 28
NCBI Official Symbol:ADAM28  
NCBI Official Synonym Symbols:MDCL; MDC-L; eMDCII; ADAM 28; eMDC II  
NCBI Protein Information:disintegrin and metalloproteinase domain-containing protein 28
UniProt Protein Name:Disintegrin and metalloproteinase domain-containing protein 28
UniProt Synonym Protein Names:Epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II; eMDC II; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L; MDC-L
UniProt Gene Name:ADAM28  
Anti-ADAM28 Antibody (CAB14849)Western blot analysis of extracts of various cell lines, using ADAM28 Rabbit pAb (CAB14849) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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