Signal Transduction Antibodies 3
Anti-ADAM17 Antibody (CAB0821)
- SKU:
- CAB0821
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-ADAM17 Antibody |
| Antibody SKU: | CAB0821 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 700 to the C-terminus of human ADAM17 (NP_003174.3). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, Jurkat, Mouse brain, Mouse heart, Rat brain, Rat testis |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 700 to the C-terminus of human ADAM17 (NP_003174.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KQYE SLSL FHPS NVEM LSSM DSAS VRII KPFP APQT PGRL QPAP VIPS APAA PKLD HQRM DTIQ EDPS TDSH MDED GFEK DPFP NSST AAKS FEDL TDHP VTRS EKAA SFKL QRQN RVDS KETE C |
| Gene ID: | 6868 |
| Uniprot: | P78536 |
| Cellular Location: | Membrane, Single-pass type I membrane protein |
| Calculated MW: | 78kDa/93kDa |
| Observed MW: | 115kDa |
| Synonyms: | ADAM17, ADAM18, CD156B, CSVP, NISBD, NISBD1, TACE |
| Background: | This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature protease. The encoded protease functions in the ectodomain shedding of tumor necrosis factor-alpha, in which soluble tumor necrosis factor-alpha is released from the membrane-bound precursor. This protease also functions in the processing of numerous other substrates, including cell adhesion proteins, cytokine and growth factor receptors and epidermal growth factor (EGF) receptor ligands. The encoded protein also plays a prominent role in the activation of the Notch signaling pathway. Elevated expression of this gene has been observed in specific cell types derived from psoriasis, rheumatoid arthritis, multiple sclerosis and Crohn's disease patients, suggesting that the encoded protein may play a role in autoimmune disease. |
| UniProt Protein Function: | TACE: a type I membrane protein with disintegrin and metalloprotease activity. An ubiquitously expressed ectoenzyme of peptidase family M12B. Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Possesses a narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-|-Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha) to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, and the amyloid precursor protein. Also involved in the activation of Notch pathway. Binds 1 zinc ion per subunit. Two splice variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; EC 3.4.24.86; Protease; Membrane protein, integral Chromosomal Location of Human Ortholog: 2p25 Cellular Component: cell surface; focal adhesion; membrane; integral to plasma membrane; apical plasma membrane; cytoplasm; plasma membrane; intercellular junction; actin cytoskeleton; lipid raft Molecular Function:integrin binding; protein binding; interleukin-6 receptor binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; Notch binding; SH3 domain binding; PDZ domain binding Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; cell motility involved in cell locomotion; neutrophil mediated immunity; T cell differentiation in the thymus; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; proteolysis; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; extracellular matrix disassembly; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; negative regulation of interleukin-8 production; germinal center formation; cell adhesion; response to drug; spleen development; epidermal growth factor receptor signaling pathway; Notch signaling pathway; membrane protein intracellular domain proteolysis; response to high density lipoprotein stimulus; membrane protein ectodomain proteolysis; positive regulation of transforming growth factor beta receptor signaling pathway; Notch receptor processing; positive regulation of cell motility; positive regulation of cell growth; positive regulation of chemokine production; collagen catabolic process; regulation of mast cell apoptosis; PMA-inducible membrane protein ectodomain proteolysis; B cell differentiation; response to hypoxia; cell adhesion mediated by integrin; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; wound healing, spreading of epidermal cells; positive regulation of cell migration Disease: Inflammatory Skin And Bowel Disease, Neonatal, 1 |
| NCBI Summary: | This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P78536 |
| NCBI GenInfo Identifier: | 73747889 |
| NCBI Gene ID: | 6868 |
| NCBI Accession: | NP_003174.3 |
| UniProt Secondary Accession: | P78536,O60226, |
| UniProt Related Accession: | P78536 |
| Molecular Weight: | 93,021 Da |
| NCBI Full Name: | disintegrin and metalloproteinase domain-containing protein 17 preproprotein |
| NCBI Synonym Full Names: | ADAM metallopeptidase domain 17 |
| NCBI Official Symbol: | ADAM17 |
| NCBI Official Synonym Symbols: | CSVP; TACE; NISBD; ADAM18; CD156B |
| NCBI Protein Information: | disintegrin and metalloproteinase domain-containing protein 17; TNF-alpha convertase; snake venom-like protease; TNF-alpha converting enzyme; ADAM metallopeptidase domain 18; tumor necrosis factor, alpha, converting enzyme |
| UniProt Protein Name: | Disintegrin and metalloproteinase domain-containing protein 17 |
| UniProt Synonym Protein Names: | Snake venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme |
| Protein Family: | Disintegrin and metalloproteinase domain-containing protein |
| UniProt Gene Name: | ADAM17 |
| UniProt Entry Name: | ADA17_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ADAM17 antibody (CAB0821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded rat heart using ADAM17 antibody (CAB0821) at dilution of 1:100 (40x lens). |
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