Cell Biology Antibodies 1
Anti-ADA Antibody (CAB1019)
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- SKU:
- CAB1019
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ADA Antibody |
| Antibody SKU: | CAB1019 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-363 of human ADA (NP_000013.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, Jurkat, U-251MG, DU145, Mouse spleen, Mouse intestine, Mouse stomach |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-363 of human ADA (NP_000013.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAQT PAFD KPKV ELHV HLDG SIKP ETIL YYGR RRGI ALPA NTAE GLLN VIGM DKPL TLPD FLAK FDYY MPAI AGCR EAIK RIAY EFVE MKAK EGVV YVEV RYSP HLLA NSKV EPIP WNQA EGDL TPDE VVAL VGQG LQEG ERDF GVKA RSIL CCMR HQPN WSPK VVEL CKKY QQQT VVAI DLAG DETI PGSS LLPG HVQA YQEA VKSG IHRT VHAG EVGS AEVV KEAV DILK TERL GHGY HTLE DQAL YNRL RQEN MHFE ICPW SSYL TGAW KPDT EHAV IRLK NDQA NYSL NTDD PLIF KSTL DTDY QMTK RDMG FTEE EFKR LNIN AAKS SFLP EDEK RELL DLLY KAYG MPPS ASAG QNL |
| Gene ID: | 100 |
| Uniprot: | P00813 |
| Cellular Location: | Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle lumen, Extracellular side, Peripheral membrane protein |
| Calculated MW: | 40kDa |
| Observed MW: | 41kDa |
| Synonyms: | ADA |
| Background: | This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. |
| UniProt Protein Function: | ADA: a enzyme that converts adenosine + H2O into inosine + NH3. Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues. Genetic ADA deficiencies are a cause of autosomal recessive severe combined immuno-deficiency (SCID). Hereditary hemolytic anemia is caused by expression levels in erythrocytes 50-70 times greater than the norm. |
| UniProt Protein Details: | Protein type:Hydrolase; Nucleotide Metabolism - purine; EC 3.5.4.4 Chromosomal Location of Human Ortholog: 20q13.12 Cellular Component: cell surface; cytoplasm; cytosol; external side of plasma membrane; lysosome; membrane; plasma membrane Molecular Function:adenosine deaminase activity; protein binding; zinc ion binding Biological Process: adenosine catabolic process; hypoxanthine salvage; inosine biosynthetic process; negative regulation of adenosine receptor signaling pathway; purine nucleotide salvage; purine salvage; regulation of cell-cell adhesion mediated by integrin; response to hypoxia; T cell activation Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-negative, Nk Cell-negative, Due To Adenosine Deaminase Deficiency |
| NCBI Summary: | This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P00813 |
| NCBI GenInfo Identifier: | 113339 |
| NCBI Gene ID: | 100 |
| NCBI Accession: | P00813.3 |
| UniProt Secondary Accession: | P00813,Q53F92, Q6LA59, |
| UniProt Related Accession: | P00813 |
| Molecular Weight: | 40,764 Da |
| NCBI Full Name: | Adenosine deaminase |
| NCBI Synonym Full Names: | adenosine deaminase |
| NCBI Official Symbol: | ADA |
| NCBI Protein Information: | adenosine deaminase |
| UniProt Protein Name: | Adenosine deaminase |
| UniProt Synonym Protein Names: | Adenosine aminohydrolase |
| Protein Family: | ADAM |
| UniProt Gene Name: | ADA |
| UniProt Entry Name: | ADA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ADA antibody (CAB1019) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of C6 cells using ADA antibody (CAB1019) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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