Name: Anti-ACTR3B Antibody (CAB7824)
Brand: Cell Biology Antibodies 11
SKU: CAB7824
Availability: OutOfStock

Antibody Name:	Anti-ACTR3B Antibody
Antibody SKU:	CAB7824
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 189-418 of human ACTR3B (NP_065178.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	HeLa, NCI-H125, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 189-418 of human ACTR3B (NP_065178.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	CIKH IPIA GRDI TYFI QQLL RERE VGIP PEQS LETA KAIK EKYC YICP DIVK EFAK YDVD PRKW IKQY TGIN AINQ KKFV IDVG YERF LGPE IFFH PEFA NPDF MESI SDVV DEVI QNCP IDVR RPLY KNVV LSGG STMF RDFG RRLQ RDLK RVVD ARLR LSEE LSGG RIKP KPVE VQVV THHM QRYA VWFG GSML ASTP EFFQ VCHT KKDY EEYG PSIC RHNP VFGV MS
Gene ID:	57180
Uniprot:	Q9P1U1
Cellular Location:	Cell projection, Cytoplasm, cytoskeleton
Calculated MW:	37kDa/39kDa/47kDa
Observed MW:	48kDa

Synonyms:

ACTR3B, ARP11, ARP3BETA

Background:

This gene encodes a member of the actin-related proteins (ARP), which form multiprotein complexes and share 35-55% amino acid identity with conventional actin. The protein encoded by this gene may have a regulatory role in the actin cytoskeleton and induce cell-shape change and motility. Pseudogenes of this gene are located on chromosomes 2, 4, 10, 16, 22 and Y. Alternative splicing results in multiple transcript variants and protein isoforms.

UniProt Protein Function:	ACTR3B: Plays a role in the organization of the actin cytoskeleton. May function as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. May decrease the metastatic potential of tumors. Belongs to the actin family. ARP3 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 7q36.1 Cellular Component: Arp2/3 protein complex; cell projection; cytoplasm Molecular Function:actin binding; ATP binding
NCBI Summary:	This gene encodes a member of the actin-related proteins (ARP), which form multiprotein complexes and share 35-55% amino acid identity with conventional actin. The protein encoded by this gene may have a regulatory role in the actin cytoskeleton and induce cell-shape change and motility. Pseudogenes of this gene are located on chromosomes 2, 4, 10, 16, 22 and Y. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jul 2012]
UniProt Code:	Q9P1U1
NCBI GenInfo Identifier:	74753111
NCBI Gene ID:	57180
NCBI Accession:	Q9P1U1.1
UniProt Secondary Accession:	Q9P1U1,Q7Z526, Q96BT2, A8MTG1, B4DFW4,
UniProt Related Accession:	Q9P1U1
Molecular Weight:	418
NCBI Full Name:	Actin-related protein 3B
NCBI Synonym Full Names:	ARP3 actin-related protein 3 homolog B (yeast)
NCBI Official Symbol:	ACTR3B
NCBI Official Synonym Symbols:	ARP11; ARP3BETA
NCBI Protein Information:	actin-related protein 3B; ARP3-beta; actin-like protein 3B; actin-related protein ARP4; actin-related protein Arp11; actin-related protein 3-beta
UniProt Protein Name:	Actin-related protein 3B
UniProt Synonym Protein Names:	ARP3-beta; Actin-like protein 3B; Actin-related protein ARP4
Protein Family:	Actin-related protein
UniProt Gene Name:	ACTR3B
UniProt Entry Name:	ARP3B_HUMAN

Western blot analysis of extracts of various cell lines, using ACTR3B antibody (CAB7824) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

ACTR3B Antibody (PACO07592)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)