Antibody Name:	Anti-ACTR1B Antibody
Antibody SKU:	CAB15773
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human ACTR1B (NP_005726.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse thymus, Mouse brain, Mouse heart, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human ACTR1B (NP_005726.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MESY DIIA NQPV VIDN GSGV IKAG FAGD QIPK YCFP NYVG RPKH MRVM AGAL EGDL FIGP KAEE HRGL LTIR YPME HGVV RDWN DMER IWQY VYSK DQLQ TFSE E
Gene ID:	10120
Uniprot:	P42025
Cellular Location:	Cytoplasm, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	42kDa
Observed MW:	42kDa

Synonyms:

ACTR1B, ARP1B, CTRN2, PC3

Background:

This gene encodes a 42.3 kD subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like ACTR1A, is an actin-related protein. These two proteins, which are of equal length and share 90% amino acid identity, are present in a constant ratio of approximately 1:15 in the dynactin complex.

UniProt Protein Function:	ACTR1B: Component of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome. Belongs to the actin family. ARP1 subfamily.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 2q11.2 Cellular Component: centrosome; dynactin complex; membrane; cytoplasm; cytosol Molecular Function:protein binding; ATP binding Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II
NCBI Summary:	This gene encodes a 42.3 kD subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like ACTR1A, is an actin-related protein. These two proteins, which are of equal length and share 90% amino acid identity, are present in a constant ratio of approximately 1:15 in the dynactin complex. [provided by RefSeq, Aug 2008]
UniProt Code:	P42025
NCBI GenInfo Identifier:	11342680
NCBI Gene ID:	10120
NCBI Accession:	NP_005726.1
UniProt Secondary Accession:	P42025,Q53SK5, Q9BRB7, D3DVH2,
UniProt Related Accession:	P42025
Molecular Weight:	42,293 Da
NCBI Full Name:	beta-centractin
NCBI Synonym Full Names:	ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
NCBI Official Symbol:	ACTR1B
NCBI Official Synonym Symbols:	PC3; ARP1B; CTRN2
NCBI Protein Information:	beta-centractin; centractin beta; actin-related protein 1B
UniProt Protein Name:	Beta-centractin
UniProt Synonym Protein Names:	Actin-related protein 1B; ARP1B
Protein Family:	Beta-centractin
UniProt Gene Name:	ACTR1B
UniProt Entry Name:	ACTY_HUMAN

	Western blot analysis of extracts of various cell lines, using ACTR1B Rabbit pAb (CAB15773) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded Mouse kidney using ACTR1B Rabbit pAb (CAB15773) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat testis using ACTR1B Rabbit pAb (CAB15773) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human colon carcinoma using ACTR1B Rabbit pAb (CAB15773) at dilution of 1:100 (40x lens).