Name: Anti-ACTR1A Antibody (CAB6515)
Brand: Cell Biology Antibodies 10
SKU: CAB6515
Availability: OutOfStock

Antibody Name:	Anti-ACTR1A Antibody
Antibody SKU:	CAB6515
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 187-376 of human ACTR1A (NP_005727.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, Mouse skeletal muscle, Mouse spleen, Mouse lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 187-376 of human ACTR1A (NP_005727.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GRDV SRFL RLYL RKEG YDFH SSSE FEIV KAIK ERAC YLSI NPQK DETL ETEK AQYY LPDG STIE IGPS RFRA PELL FRPD LIGE ESEG IHEV LVFA IQKS DMDL RRTL FSNI VLSG GSTL FKGF GDRL LSEV KKLA PKDV KIRI SAPQ ERLY STWI GGSI LASL DTFK KMWV SKKE YEED GARS IHRK TF
Gene ID:	10121
Uniprot:	P61163
Cellular Location:	Cytoplasm, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	42kDa
Observed MW:	43kDa

Synonyms:

ACTR1A, ARP1, Arp1A, CTRN1

Background:

This gene encodes a 42.6 kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 8-13 copies per dynactin molecule, and is the most abundant molecule in the dynactin complex. It is an actin-related protein, and is approximately 60% identical at the amino acid level to conventional actin.

UniProt Protein Function:	ACTR1A: Component of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome. Belongs to the actin family. ARP1 subfamily.
UniProt Protein Details:	Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 10q24.32 Cellular Component: centrosome; cytoplasm; cytosol; microtubule associated complex; microtubule cytoskeleton Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; G2/M transition of mitotic cell cycle; vesicle-mediated transport
NCBI Summary:	This gene encodes a 42.6 kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 8-13 copies per dynactin molecule, and is the most abundant molecule in the dynactin complex. It is an actin-related protein, and is approximately 60% identical at the amino acid level to conventional actin. [provided by RefSeq, Jul 2008]
UniProt Code:	P61163
NCBI GenInfo Identifier:	47117651
NCBI Gene ID:	10121
NCBI Accession:	P61163.1
UniProt Secondary Accession:	P61163,P42024, B2R6B0,
UniProt Related Accession:	P61163,AAB23391
Molecular Weight:	42,614 Da
NCBI Full Name:	Alpha-centractin
NCBI Synonym Full Names:	ARP1 actin-related protein 1 homolog A, centractin alpha
NCBI Official Symbol:	ACTR1A
NCBI Official Synonym Symbols:	ARP1; Arp1A; CTRN1
NCBI Protein Information:	alpha-centractin
UniProt Protein Name:	Alpha-centractin
UniProt Synonym Protein Names:	ARP1; Actin-RPV; Centrosome-associated actin homolog
Protein Family:	Alpha-centractin
UniProt Gene Name:	ACTR1A
UniProt Entry Name:	ACTZ_HUMAN

Western blot analysis of extracts of various cell lines, using ACTR1A antibody (CAB6515) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

ACTR1A Antibody (PACO07588)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)