Cell Biology Antibodies 7
Anti-ACTN4 Antibody (CAB16310)
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- SKU:
- CAB16310
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ACTN4 Antibody |
| Antibody SKU: | CAB16310 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 570-650 of human ACTN4 (NP_004915.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, Raji, Mouse liver, Mouse kidney, Mouse heart, Rat lung, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 570-650 of human ACTN4 (NP_004915.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HDQF KSTL PDAD RERE AILA IHKE AQRI AESN HIKL SGSN PYTT VTPQ IINS KWEK VQQL VPKR DHAL LEEQ SKQQ SNEH L |
| Gene ID: | 81 |
| Uniprot: | O43707 |
| Cellular Location: | Cell junction, Cytoplasm, Nucleus |
| Calculated MW: | 59kDa/79kDa/104kDa |
| Observed MW: | 105kDa |
| Synonyms: | ACTN4, ACTININ-4, FSGS, FSGS1 |
| Background: | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. |
| UniProt Protein Function: | ACTN4: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Defects in ACTN4 are the cause of focal segmental glomerulosclerosis type 1 (FSGS1). A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. Belongs to the alpha-actinin family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 19q13 Cellular Component: cortical cytoskeleton; extracellular space; neuron projection; focal adhesion; protein complex; perinuclear region of cytoplasm; cytoplasm; stress fiber; extracellular region; intercellular junction; pseudopodium; Z disc; ribonucleoprotein complex; nucleus Molecular Function:actin filament binding; integrin binding; protein binding; protein homodimerization activity; ligand-dependent nuclear receptor transcription coactivator activity; retinoic acid receptor binding; nucleoside binding; protein N-terminus binding; calcium ion binding; actin binding; nuclear hormone receptor binding Biological Process: regulation of apoptosis; retinoic acid receptor signaling pathway; platelet activation; actin filament bundle formation; protein transport; platelet degranulation; response to hypoxia; negative regulation of cell motility; positive regulation of cell motility; positive regulation of sodium:hydrogen antiporter activity; positive regulation of pinocytosis; blood coagulation Disease: Focal Segmental Glomerulosclerosis 1 |
| NCBI Summary: | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O43707 |
| NCBI GenInfo Identifier: | 13123943 |
| NCBI Gene ID: | 81 |
| NCBI Accession: | O43707.2 |
| UniProt Secondary Accession: | O43707,O76048, A4K467, D6PXK4, |
| UniProt Related Accession: | O43707 |
| Molecular Weight: | 911 |
| NCBI Full Name: | Alpha-actinin-4 |
| NCBI Synonym Full Names: | actinin, alpha 4 |
| NCBI Official Symbol: | ACTN4 |
| NCBI Official Synonym Symbols: | FSGS; FSGS1; ACTININ-4 |
| NCBI Protein Information: | alpha-actinin-4; actinin alpha4 isoform; non-muscle alpha-actinin 4; F-actin cross-linking protein; focal segmental glomerulosclerosis 1 |
| UniProt Protein Name: | Alpha-actinin-4 |
| UniProt Synonym Protein Names: | F-actin cross-linking protein; Non-muscle alpha-actinin 4 |
| Protein Family: | Alpha-actinin |
| UniProt Gene Name: | ACTN4 |
| UniProt Entry Name: | ACTN4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ACTN4 antibody (CAB16310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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