Cell Biology Antibodies 3
Anti-ACTN3 Antibody (CAB12864)
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- SKU:
- CAB12864
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ACTN3 Antibody |
| Antibody SKU: | CAB12864 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 580-630 of human ACTN3 (NP_001095.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, Mouse skeletal muscle, Rat skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 580-630 of human ACTN3 (NP_001095.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GAIM GIQG EIQK ICQT YGLR PCST NPYI TLSP QDIN TKWD MVRK LVPS CDQ |
| Gene ID: | 89 |
| Uniprot: | Q08043 |
| Cellular Location: | |
| Calculated MW: | 103kDa |
| Observed MW: | 103kDa |
| Synonyms: | ACTN3 |
| Background: | This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status. |
| UniProt Protein Function: | ACTN3: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Belongs to the alpha-actinin family. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 11q13.2 Cellular Component: actin filament; cytosol; focal adhesion; intracellular; pseudopodium Molecular Function:integrin binding; protein binding; protein homodimerization activity; structural constituent of muscle Biological Process: focal adhesion formation; muscle filament sliding; negative regulation of glycolysis; positive regulation of skeletal muscle growth; regulation of the force of skeletal muscle contraction; response to denervation involved in regulation of muscle adaptation; skeletal muscle atrophy; transition between fast and slow fiber |
| NCBI Summary: | This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status. [provided by RefSeq, Feb 2014] |
| UniProt Code: | Q08043 |
| NCBI GenInfo Identifier: | 215273967 |
| NCBI Gene ID: | 89 |
| NCBI Accession: | Q08043.2 |
| UniProt Secondary Accession: | Q08043,Q4KKV2, A6NP77, |
| UniProt Related Accession: | Q08043 |
| Molecular Weight: | 103kDa |
| NCBI Full Name: | Alpha-actinin-3 |
| NCBI Synonym Full Names: | actinin alpha 3 (gene/pseudogene) |
| NCBI Official Symbol: | ACTN3 |
| NCBI Protein Information: | alpha-actinin-3 |
| UniProt Protein Name: | Alpha-actinin-3 |
| UniProt Synonym Protein Names: | Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein |
| Protein Family: | Alpha-actinin |
| UniProt Gene Name: | ACTN3 |
 | Western blot analysis of extracts of various cell lines, using ACTN3 antibody (CAB12864) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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