Antibody Name:	Anti-ACTL6B Antibody
Antibody SKU:	CAB7108
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ACTL6B (NP_057272.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, 22Rv1, BT-474, HeLa, HepG2, Mouse brain, Mouse skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ACTL6B (NP_057272.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSGG VYGG DEVG ALVF DIGS FSVR AGYA GEDC PKAD FPTT VGLL AAEE GGGL ELEG DKEK KGKI FHID TNAL HVPR DGAE VMSP LKNG MIED WECF RAIL DHTY SKHV KSEP NLHP VLMS EAPW NTRA KREK LTEL MFEQ YNIP AFFL CKTA VLTA FANG RSTG LVLD SGAT HTTA IPVH DGYV LQQG IVKS PLAG DFIS MQCR ELFQ EMAI DIIP PYMI AAKE PVRE GAPP NWKK KEKL PQVS KSWH NYMC NEVI QDFQ ASVL QVSD SPYD EQVA AQMP
Gene ID:	51412
Uniprot:	O94805
Cellular Location:	Nucleus
Calculated MW:	46kDa
Observed MW:	47-55kDa

Synonyms:

ACTL6B, ACTL6, BAF53B, arpNalpha

Background:

The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	BAF53B: a protein of the actin family involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for maximal ATPase activity of the helicase SMARCA4 and for association of the SMARCA4 containing remodelling complex BAF with chromatin/nuclear matrix.
UniProt Protein Details:	Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 7q22 Cellular Component: SWI/SNF complex; nucleolus; nucleus Molecular Function:structural constituent of cytoskeleton; transcription coactivator activity Biological Process: chromatin remodeling; regulation of transcription from RNA polymerase II promoter; nervous system development; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent
NCBI Summary:	The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain. [provided by RefSeq, Jul 2008]
UniProt Code:	O94805
NCBI GenInfo Identifier:	23396462
NCBI Gene ID:	51412
NCBI Accession:	O94805.1
UniProt Secondary Accession:	O94805,O75421, A4D2D0,
UniProt Related Accession:	O94805
Molecular Weight:	426
NCBI Full Name:	Actin-like protein 6B
NCBI Synonym Full Names:	actin-like 6B
NCBI Official Symbol:	ACTL6B
NCBI Official Synonym Symbols:	ACTL6; BAF53B
NCBI Protein Information:	actin-like protein 6B; arpNalpha; hArpN alpha; actin-like 6; BRG1-associated factor 53B; actin-related protein Baf53b; 53 kDa BRG1-associated factor B
UniProt Protein Name:	Actin-like protein 6B
UniProt Synonym Protein Names:	53 kDa BRG1-associated factor B; Actin-related protein Baf53b; ArpNalpha; BRG1-associated factor 53B; BAF53B
Protein Family:	Actin-like protein
UniProt Gene Name:	ACTL6B
UniProt Entry Name:	ACL6B_HUMAN

	Western blot analysis of extracts of various cell lines, using ACTL6B antibody (CAB7108) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 40s.
	Immunohistochemistry of paraffin-embedded human kidney using ACTL6B antibody (CAB7108) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney cancer using ACTL6B antibody (CAB7108) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse jejunum using ACTL6B antibody (CAB7108) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A-549 cells using ACTL6B antibody (CAB7108).