Anti-ACTA2/ACTG2 Antibody (MACO0472)
- SKU:
- MACO0472
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Mouse
- Isotype:
- IgG2a, Kappa
- Applications:
- ELISA
- Applications:
- IHC
- Antibody Type:
- Monoclonal Antibody
- Conjugation:
- Unconjugated
Description
| Product Name: | Anti-ACTA2/ACTG2 Antibody |
| Product Sku: | MACO0472 |
| Size: | 50ul |
| Host Species: | Mouse |
| Tested Applications: | ELISA, IHC |
| Recommended Dilutions: | IHC:1:20-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Synthesized peptide derived from human Actin, smooth muscle (SMA) |
| Form: | Liquid |
| Storage Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification Method: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Clonality: | Monoclonal |
| Isotype: | IgG2a, Kappa |
| Conjugate: | Non-conjugated |
| Background: | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| Synonyms: | Actin, aortic smooth muscle (Alpha-actin-2) (Cell growth-inhibiting gene 46 protein) [Cleaved into: Actin, aortic smooth muscle, intermediate form], ACTA2, ACTSA ACTVS |
 | IHC image of MACO0472 diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| UniProt Protein Function: | ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family. |
| UniProt Protein Details: | Protein type:Contractile; Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 10q23.3 Cellular Component: extracellular space; smooth muscle contractile fiber; protein complex; cytoplasm; cytosol; actin cytoskeleton Molecular Function:protein kinase binding; ATP binding Biological Process: muscle contraction; response to virus; regulation of blood pressure; vascular smooth muscle contraction Disease: Moyamoya Disease 5; Multisystemic Smooth Muscle Dysfunction Syndrome; Aortic Aneurysm, Familial Thoracic 6 |
| NCBI Summary: | The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008] |
| UniProt Code: | P62736 |
| NCBI GenInfo Identifier: | 51316972 |
| NCBI Gene ID: | 59 |
| NCBI Accession: | P62736.1 |
| UniProt Secondary Accession: | P62736,P03996, P04108, Q6FI19, B2R8A4, |
| UniProt Related Accession: | P62736 |
| Molecular Weight: | 42,009 Da |
| NCBI Full Name: | Actin, aortic smooth muscle |
| NCBI Synonym Full Names: | actin, alpha 2, smooth muscle, aorta |
| NCBI Official Symbol: | ACTA2 |
| NCBI Official Synonym Symbols: | AAT6; ACTSA; MYMY5 |
| NCBI Protein Information: | actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein |
| UniProt Protein Name: | Actin, aortic smooth muscle |
| UniProt Synonym Protein Names: | Alpha-actin-2; Cell growth-inhibiting gene 46 protein |
| Protein Family: | Actin |
| UniProt Gene Name: | ACTA2 |
| UniProt Entry Name: | ACTA_HUMAN |
