Cell Biology Antibodies 5
Anti-ACSS1 Antibody (CAB15007)
(No reviews yet)
Write a Review
- SKU:
- CAB15007
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ACSS1 Antibody |
| Antibody SKU: | CAB15007 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 340-689 of human ACSS1 (NP_115890.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, A-549, Mouse heart, Mouse testis, Mouse kidney, Rat spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 340-689 of human ACSS1 (NP_115890.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ITGH SYVV YGPL CNGA TSVL FEST PVYP NAGR YWET VERL KINQ FYGA PTAV RLLL KYGD AWVK KYDR SSLR TLGS VGEP INCE AWEW LHRV VGDS RCTL VDTW WQTE TGGI CIAP RPSE EGAE ILPA MAMR PFFG IVPV LMDE KGSV VEGS NVSG ALCI SQAW PGMA RTIY GDHQ RFVD AYFK AYPG YYFT GDGA YRTE GGYY QITG RMDD VINI SGHR LGTA EIED AIAD HPAV PESA VIGY PHDI KGEA AFAF IVVK DSAG DSDV VVQE LKSM VATK IAKY AVPD EILV VKRL PKTR SGKV MRRL LRKI ITSE AQEL GDTT TLED PSII AEIL SVYQ KCKD KQAA AK |
| Gene ID: | 84532 |
| Uniprot: | Q9NUB1 |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 62kDa/74kDa |
| Observed MW: | 75kDa |
| Synonyms: | ACSS1, ACAS2L, ACECS1, AceCS2L |
| Background: | This gene encodes a mitochondrial acetyl-CoA synthetase enzyme. A similar protein in mice plays an important role in the tricarboxylic acid cycle by catalyzing the conversion of acetate to acetyl CoA. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | ACSS1: Important for maintaining normal body temperature during fasting and for energy homeostasis. Essential for energy expenditure under ketogenic conditions. Converts acetate to acetyl-CoA so that it can be used for oxidation through the tricarboxylic cycle to produce ATP and CO(2). Belongs to the ATP-dependent AMP-binding enzyme family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 6.2.1.1; Mitochondrial; Carbohydrate Metabolism - propanoate; Ligase Chromosomal Location of Human Ortholog: 20p11.21 Cellular Component: mitochondrial matrix Molecular Function:acetate-CoA ligase activity; AMP binding; ATP binding; protein binding Biological Process: acetate biosynthetic process; acetyl-CoA biosynthetic process; acetyl-CoA biosynthetic process from acetate; ethanol oxidation; propionate biosynthetic process; xenobiotic metabolic process |
| NCBI Summary: | This gene encodes a mitochondrial acetyl-CoA synthetase enzyme. A similar protein in mice plays an important role in the tricarboxylic acid cycle by catalyzing the conversion of acetate to acetyl CoA. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] |
| UniProt Code: | Q9NUB1 |
| NCBI GenInfo Identifier: | 30172968 |
| NCBI Gene ID: | 84532 |
| NCBI Accession: | Q9NUB1.2 |
| UniProt Secondary Accession: | Q9NUB1,Q5TF42, Q8IV99, Q8N234, Q96JI1, Q96JX6, Q9NU28 B3KXL2, B4DJZ3, D3DW48, F5H6F4, F8W7Y1, |
| UniProt Related Accession: | Q9NUB1 |
| Molecular Weight: | 62,688 Da |
| NCBI Full Name: | Acetyl-coenzyme A synthetase 2-like, mitochondrial |
| NCBI Synonym Full Names: | acyl-CoA synthetase short-chain family member 1 |
| NCBI Official Symbol: | ACSS1 |
| NCBI Official Synonym Symbols: | ACAS2L; ACECS1; AceCS2L |
| NCBI Protein Information: | acetyl-coenzyme A synthetase 2-like, mitochondrial |
| UniProt Protein Name: | Acetyl-coenzyme A synthetase 2-like, mitochondrial |
| UniProt Synonym Protein Names: | Acetate--CoA ligase 2; Acetyl-CoA synthetase 2; AceCS2; Acyl-CoA synthetase short-chain family member 1 |
| Protein Family: | Acetyl-coenzyme A synthetase |
| UniProt Gene Name: | ACSS1 |
| UniProt Entry Name: | ACS2L_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ACSS1 antibody (CAB15007) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded Human colon carcinoma using ACSS1 Rabbit pAb (CAB15007) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of L929 cells using ACSS1 antibody (CAB15007) at dilution of 1:100. Blue: DAPI for nuclear staining. |
View AllClose
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक
Human Anti-DSG3 antibody(anti-Desmoglein-3 antibody) ELISA Kit
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक
Human anti-ASGPR antibody(anti asialoglyco protein receptor antibody)ELISA Kit
OverviewProduct Name:Human anti-ASGPR antibody(anti asialoglyco protein receptor antibody)ELISA KitProduct Code:AEFI00360Size:96TAlias:a
