Cell Biology Antibodies 7

Anti-ACPP Antibody (CAB1937)

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SKU:
CAB1937
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-ACPP Antibody
Antibody SKU:CAB1937
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-300 of human ACPP (NP_001090.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HL-60, MCF7, Mouse lung, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-300 of human ACPP (NP_001090.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LVFR HGDR SPID TFPT DPIK ESSW PQGF GQLT QLGM EQHY ELGE YIRK RYRK FLNE SYKH EQVY IRST DVDR TLMS AMTN LAAL FPPE GVSI WNPI LLWQ PIPV HTVP LSED QLLY LPFR NCPR FQEL ESET LKSE EFQK RLHP YKDF IATL GKLS GLHG QDLF GIWS KVYD PLYC ESVH NFTL PSWA TEDT MTKL RELS ELSL LSLY GIHK QKEK SRLQ GGVL VNEI LNHM KRAT QIPS YKKL IMYS AHDT TVSG LQMA L
Gene ID:55
Uniprot:P15309
Cellular Location:Cell membrane, Lysosome membrane, Secreted, Single-pass type I membrane protein
Calculated MW:40kDa/44kDa/48kDa
Observed MW:43kDa
Synonyms:ACPP, 5'-NT, ACP-3, ACP3
Background:This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway.
UniProt Protein Function:ACPP: A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Belongs to the histidine acid phosphatase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.1.3.2; Cofactor and Vitamin Metabolism - riboflavin; Phosphatase; EC 3.1.3.5; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: multivesicular body; extracellular space; Golgi cisterna; apical part of cell; lysosomal membrane; plasma membrane; integral to membrane; vesicle membrane; intracellular; nucleus; secretory granule; filopodium

Molecular Function:5'-nucleotidase activity; choline binding; identical protein binding; acid phosphatase activity; protein homodimerization activity; phosphoric monoester hydrolase activity; thiamin phosphate phosphatase activity

Biological Process: thiamin metabolic process; dephosphorylation; positive regulation of adenosine receptor signaling pathway; nucleotide metabolic process; regulation of sensory perception of pain; adenosine metabolic process; purine base metabolic process

NCBI Summary:This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008]
UniProt Code:P15309
NCBI GenInfo Identifier:130730
NCBI Gene ID:55
NCBI Accession:P15309.3
UniProt Related Accession:P15309
Molecular Weight:
NCBI Full Name:Prostatic acid phosphatase
NCBI Synonym Full Names:acid phosphatase, prostate
NCBI Official Symbol:ACPP  
NCBI Official Synonym Symbols:ACP3; 5'-NT; ACP-3  
NCBI Protein Information:prostatic acid phosphatase
UniProt Protein Name:Prostatic acid phosphatase
UniProt Synonym Protein Names:5'-nucleotidase (EC:3.1.3.5); 5'-NT; Ecto-5'-nucleotidase; Thiamine monophosphatase; TMPase
UniProt Gene Name:ACPP  
UniProt Entry Name:PPAP_HUMAN
Anti-ACPP Antibody (CAB1937)Western blot analysis of extracts of various cell lines, using ACPP antibody (CAB1937) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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