Cell Biology Antibodies 7

Anti-ACP5 Antibody (CAB16338)

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SKU:
CAB16338
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-ACP5 Antibody
Antibody SKU:CAB16338
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 76-325 of human ACP5 (NP_001602.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Mouse
Positive Samples:Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 76-325 of human ACP5 (NP_001602.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TGVQ DIND KRFQ ETFE DVFS DRSL RKVP WYVL AGNH DHLG NVSA QIAY SKIS KRWN FPSP FYRL HFKI PQTN VSVA IFML DTVT LCGN SDDF LSQQ PERP RDVK LART QLSW LKKQ LAAA REDY VLVA GHYP VWSI AEHG PTHC LVKQ LRPL LATY GVTA YLCG HDHN LQYL QDEN GVGY VLSG AGNF MDPS KRHQ RKVP NGYL RFHY GTED SLGG FAYV EISS KEMT VTYI EASG KSLF KTRL PRRA RP
Gene ID:54
Uniprot:P13686
Cellular Location:Lysosome
Calculated MW:36kDa
Observed MW:36kDa
Synonyms:ACP5, HPAP, TRACP5a, TRACP5b, TRAP, TrATPase
Background:This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate.
UniProt Protein Function:ACP5: Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Defects in ACP5 are the cause of spondyloenchondrodysplasia with immune dysregulation (SPENCDI). A disease characterized by vertebral and metaphyseal dysplasia, spasticity with cerebral calcifications, and strong predisposition to autoimmune diseases. The skeletal dysplasia is characterized by radiolucent and irregular spondylar and metaphyseal lesions that represent islands of chondroid tissue within bone. ACP5 inactivating mutations result in a functional excess of phosphorylated osteopontin causing deregulation of osteopontin signaling and consequential autoimmune disease. Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Phosphatase; Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.2

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: lysosome; integral to membrane; cytosol

Molecular Function:acid phosphatase activity; ferric iron binding; ferrous iron binding

Biological Process: riboflavin metabolic process; negative regulation of interleukin-12 production; defense response to Gram-positive bacterium; vitamin metabolic process; dephosphorylation; response to cytokine stimulus; negative regulation of nitric oxide biosynthetic process; negative regulation of inflammatory response; response to lipopolysaccharide; negative regulation of interleukin-1 beta production; negative regulation of tumor necrosis factor production; negative regulation of superoxide release; water-soluble vitamin metabolic process; bone resorption

Disease: Spondyloenchondrodysplasia With Immune Dysregulation

NCBI Summary:This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008]
UniProt Code:P13686
NCBI GenInfo Identifier:56757583
NCBI Gene ID:54
NCBI Accession:P13686.3
UniProt Secondary Accession:P13686,Q2TAB1, Q6IAS6, Q9UCJ5, Q9UCJ6, Q9UCJ7, A8K3V2
UniProt Related Accession:P13686
Molecular Weight:325
NCBI Full Name:Tartrate-resistant acid phosphatase type 5
NCBI Synonym Full Names:acid phosphatase 5, tartrate resistant
NCBI Official Symbol:ACP5  
NCBI Official Synonym Symbols:TRAP; SPENCDI  
NCBI Protein Information:tartrate-resistant acid phosphatase type 5; TrATPase; tartrate-resistant acid ATPase
UniProt Protein Name:Tartrate-resistant acid phosphatase type 5
UniProt Synonym Protein Names:Tartrate-resistant acid ATPase; TrATPase; Type 5 acid phosphatase
Protein Family:Acyl carrier protein
UniProt Gene Name:ACP5  
UniProt Entry Name:PPA5_HUMAN
Anti-ACP5 Antibody (CAB16338)Western blot analysis of extracts of Mouse kidney, using ACP5 antibody (CAB16338) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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