Name: Anti-ACOT8 Antibody (CAB13067)
Brand: Metabolism Antibodies 1
SKU: CAB13067
Availability: OutOfStock

Antibody Name:	Anti-ACOT8 Antibody
Antibody SKU:	CAB13067
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ACOT8 (NP_005460.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	Jurkat, Raji, U-87MG, A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ACOT8 (NP_005460.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSSP QAPE DGQG CGDR GDPP GDLR SVLV TTVL NLEP LDED LFRG RHYW VPAK RLFG GQIV GQAL VAAA KSVS EDVH VHSL HCYF VRAG DPKL PVLY QVER TRTG SSFS VRSV KAVQ HGKP IFIC QASF QQAQ PSPM QHQF SMPT VPPP EELL DCET LIDQ YLRD PNLQ KRYP LALN RIAA QEVP IEIK PVNP SPLS QLQR MEPK QMFW VRAR GYIG EGDM
Gene ID:	10005
Uniprot:	O14734
Cellular Location:	Peroxisome
Calculated MW:	35kDa
Observed MW:	36kDa

Synonyms:	ACOT8, HNAACTE, NAP1, PTE-1, PTE-2, PTE1, PTE2, hACTE-III, hTE
Background:	The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells.

UniProt Protein Function:	ACOT8: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May mediate Nef-induced down-regulation of CD4. Major thioesterase in peroxisomes. Competes with BAAT (Bile acid CoA: amino acid N- acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs. May be involved in the metabolic regulation of peroxisome proliferation. Belongs to the C/M/P thioester hydrolase family.
UniProt Protein Details:	Protein type:EC 3.1.2.27; Hydrolase Chromosomal Location of Human Ortholog: 20q13.12 Cellular Component: peroxisomal matrix Molecular Function:acetyl-CoA hydrolase activity; acyl-CoA hydrolase activity; CoA hydrolase activity; palmitoyl-CoA hydrolase activity; protein binding; receptor binding Biological Process: acyl-CoA metabolic process; bile acid biosynthetic process; dicarboxylic acid catabolic process; fatty acid beta-oxidation using acyl-CoA oxidase; negative regulation of CD4 biosynthetic process; peroxisome fission
NCBI Summary:	The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010]
UniProt Code:	O14734
NCBI GenInfo Identifier:	14285685
NCBI Gene ID:	10005
NCBI Accession:	O14734.1
UniProt Secondary Accession:	O14734,O15261, Q17RX4,
UniProt Related Accession:	O14734
Molecular Weight:	35,914 Da
NCBI Full Name:	Acyl-coenzyme A thioesterase 8
NCBI Synonym Full Names:	acyl-CoA thioesterase 8
NCBI Official Symbol:	ACOT8
NCBI Official Synonym Symbols:	hTE; NAP1; PTE1; PTE2; PTE-1; PTE-2; HNAACTE; hACTE-III
NCBI Protein Information:	acyl-coenzyme A thioesterase 8
UniProt Protein Name:	Acyl-coenzyme A thioesterase 8
UniProt Synonym Protein Names:	Choloyl-coenzyme A thioesterase; HIV-Nef-associated acyl-CoA thioesterase; PTE-2; Peroxisomal acyl-coenzyme A thioester hydrolase 1; PTE-1; Peroxisomal long-chain acyl-CoA thioesterase 1; Thioesterase II; hACTE-III; hACTEIII; hTE
Protein Family:	Acyl-coenzyme A thioesterase
UniProt Gene Name:	ACOT8
UniProt Entry Name:	ACOT8_HUMAN

Western blot analysis of extracts of various cell lines, using ACOT8 antibody (CAB13067) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

ACOT8 Antibody (PACO06224)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)