Metabolism Antibodies 2
Anti-ACO2 Antibody (CAB3716)
- SKU:
- CAB3716
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-ACO2 Antibody |
| Antibody SKU: | CAB3716 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 501-780 of human ACO2 (NP_001089.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, U-87MG, K-562, NIH/3T3, Mouse brain, Rat spinal cord |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 501-780 of human ACO2 (NP_001089.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IAGT LKFN PETD YLTG TDGK KFRL EAPD ADEL PKGE FDPG QDTY QHPP KDSS GQHV DVSP TSQR LQLL EPFD KWDG KDLE DLQI LIKV KGKC TTDH ISAA GPWL KFRG HLDN ISNN LLIG AINI ENGK ANSV RNAV TQEF GPVP DTAR YYKK HGIR WVVI GDEN YGEG SSRE HAAL EPRH LGGR AIIT KSFA RIHE TNLK KQGL LPLT FADP ADYN KIHP VDKL TIQG LKDF TPGK PLKC IIKH PNGT QETI LLNH TFNE TQIE WFRA GSAL NRMK ELQQ |
| Gene ID: | 50 |
| Uniprot: | Q99798 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 85kDa |
| Observed MW: | 105kDa |
| Synonyms: | ACO2, ACONM, HEL-S-284, ICRD, OCA8, OPA9 |
| Background: | The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. |
| UniProt Protein Function: | ACO2: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. Monomer. Belongs to the aconitase/IPM isomerase family. |
| UniProt Protein Details: | Protein type:Lyase; EC 4.2.1.3; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial Chromosomal Location of Human Ortholog: 22q13.2 Cellular Component: mitochondrion; mitochondrial matrix; nucleus Molecular Function:iron ion binding; 4 iron, 4 sulfur cluster binding; aconitate hydratase activity; 3 iron, 4 sulfur cluster binding Biological Process: isocitrate metabolic process; cellular metabolic process; generation of precursor metabolites and energy; tricarboxylic acid cycle; citrate metabolic process Disease: Infantile Cerebellar-retinal Degeneration; Optic Atrophy 8 |
| NCBI Summary: | The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q99798 |
| NCBI GenInfo Identifier: | 6686275 |
| NCBI Gene ID: | 50 |
| NCBI Accession: | Q99798.2 |
| UniProt Related Accession: | Q99798 |
| Molecular Weight: | |
| NCBI Full Name: | Aconitate hydratase, mitochondrial |
| NCBI Synonym Full Names: | aconitase 2 |
| NCBI Official Symbol: | ACO2 |
| NCBI Official Synonym Symbols: | ICRD; OCA8; OPA9; ACONM; HEL-S-284 |
| NCBI Protein Information: | aconitate hydratase, mitochondrial |
| UniProt Protein Name: | Aconitate hydratase, mitochondrial |
| UniProt Synonym Protein Names: | Citrate hydro-lyase |
| Protein Family: | Aconitate hydratase |
| UniProt Gene Name: | ACO2 |
| UniProt Entry Name: | ACON_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ACO2 antibody (CAB3716) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of HeLa cells using ACO2 antibody (CAB3716) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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