Anti-Acetyl-Histone H3-K18 Antibody (CAB20735)

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SKU:
CAB20735
Product Type:
Antibody
Antibody Type:
Secondary Antibody
Host Species:
Rabbit
Isotype:
Horseradish peroxidase conjugated IgG

Description

Antibody Name:Anti-Acetyl-Histone H3-K18 Antibody (CAB20735)
Antibody SKU:CAB20735
Antibody Size:50µL, 100µL
Application:Western blotting, Immunohistochemistry, Immunofluorescence
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Host Species:Rabbit
Immunogen:A synthetic acetylated peptide corresponding to residues surrounding K18 of human Histone H3
Application:Western blotting, Immunohistochemistry, Immunofluorescence
Recommended Dilution:WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
ChIP 1:50 - 1:200
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Positive Samples:
Immunogen:A synthetic acetylated peptide corresponding to residues surrounding K18 of human Histone H3
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:Chromosome, Nucleus
Calculated MW:15kDa
Observed MW:Refer to figures
Synonyms:H3/A, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3C10, H3C11, H3C12, HIST1H3A
Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K18 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K18 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Immunofluorescence analysis of NIH/3T3 using Acetyl-Histone H3-K18 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of NIH/3T3 using Acetyl-Histone H3-K18 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 using Acetyl-Histone H3-K18 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of PC-12 using Acetyl-Histone H3-K18 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS using Acetyl-Histone H3-K18 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.Immunofluorescence analysis of U2OS using Acetyl-Histone H3-K18 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H3-K18 antibody at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H3-K18 antibody at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse lung using Acetyl-Histone H3-K18 antibody at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded mouse lung using Acetyl-Histone H3-K18 antibody at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver cancer using Acetyl-Histone H3-K18 antibody at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded human liver cancer using Acetyl-Histone H3-K18 antibody at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K18 antibody at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K18 antibody at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
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