Cell Biology Antibodies 16
Anti-Acetyl-Histone H2B-K15 Antibody (CAB15622)
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- SKU:
- CAB15622
Description
| Antibody Name: | Anti-Acetyl-Histone H2B-K15 Antibody |
| Antibody SKU: | CAB15622 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic acetylated peptide corresponding to residues surrounding K15 of human Histone H2B |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, NIH/3T3, C6 |
| Immunogen: | A synthetic acetylated peptide corresponding to residues surrounding K15 of human Histone H2B |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 3017/8349 |
| Uniprot: | |
| Cellular Location: | |
| Calculated MW: | |
| Observed MW: | 15kDa |
| Synonyms: | |
| Background: |
 | Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K15 antibody (CAB15622) at 1:1000 dilution.HeLa cells were treated by TSA (1 uM) at 37'C for 18 hours.NIH/3T3 cells were treated by TSA (1 uM) at 37'C for 18 hours.C6 cells were treated by TSA (1 uM) at 37'C for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat lung using H2B K15ac antibody (CAB15622) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using H2B K15ac antibody (CAB15622) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using H2B K15ac antibody (CAB15622) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using H2B K15ac antibody (CAB15622) at dilution of 1:100 (40x lens). |
Additional Information
Product Type: |
Antibody |
Reactivity: |
Human |
Reactivity: |
Mouse |
Reactivity: |
Rat |
Host Species: |
Rabbit |
Isotype: |
IgG |
Antibody Type: |
Polyclonal Antibody |
Research Area: |
Cell Biology |
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