Cell Biology Antibodies 6
Anti-Acetyl-Histone H2B-K12 Antibody (CAB15619)
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- SKU:
- CAB15619
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Acetyl-Histone H2B-K12 Antibody |
| Antibody SKU: | CAB15619 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic acetylated peptide around K12 of human Histone H2B (NP_003519.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, C2C12, C6 |
| Immunogen: | A synthetic acetylated peptide around K12 of human Histone H2B (NP_003519.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | APKK G |
| Gene ID: | 8349 |
| Uniprot: | Q16778 |
| Cellular Location: | Chromosome, Nucleus |
| Calculated MW: | 13kDa |
| Observed MW: | 14kDa |
| Synonyms: | GL105, H2B, H2B.1, H2BFQ, H2BGL105, H2BQ, Histone H2B, HIST2H2BE |
| Background: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity. |
| UniProt Protein Function: | H2B2E: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H2B family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 1q21.2 Cellular Component: nucleoplasm; extracellular space; nucleosome; nucleus Molecular Function:DNA binding; protein heterodimerization activity Biological Process: nucleosome assembly; defense response to Gram-positive bacterium; establishment and/or maintenance of chromatin architecture; innate immune response in mucosa; antibacterial humoral response |
| NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity. [provided by RefSeq, Aug 2015] |
| UniProt Code: | Q16778 |
| NCBI GenInfo Identifier: | 7387736 |
| NCBI Gene ID: | 8349 |
| NCBI Accession: | Q16778.3 |
| UniProt Related Accession: | Q16778 |
| Molecular Weight: | 14kDa |
| NCBI Full Name: | Histone H2B type 2-E |
| NCBI Synonym Full Names: | H2B clustered histone 21 |
| NCBI Official Symbol: | H2BC21 |
| NCBI Official Synonym Symbols: | H2B; H2BQ; GL105; H2B.1; H2BFQ; H2BGL105; HIST2H2BE |
| NCBI Protein Information: | histone H2B type 2-E |
| UniProt Protein Name: | Histone H2B type 2-E |
| UniProt Synonym Protein Names: | Histone H2B-GL105; Histone H2B.q; H2B/q |
| Protein Family: | Histone |
| UniProt Gene Name: | HIST2H2BE |
| UniProt Entry Name: | H2B2E_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K12 antibody at 1:1000 dilution. HeLa cells and C2C12 cells and C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H2B-K12 antibody at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H2B-K12 antibody at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H2B-K12 antibody at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K12 antibody at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37°C for 18 hours (top left). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K12 antibody at dilution of 1:100. HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours (top left). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K12 antibody at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours (top left). Blue: DAPI for nuclear staining. |
 | Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H2B-K12 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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