Antibody Name:	Anti-Acetyl-Histone H2A-K5 Antibody
Antibody SKU:	CAB15620
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat, Other (Wide Range)
Host Species:	Rabbit
Immunogen:	A synthetic acetylated peptide around K5 of human Histone H2A (NP_003508.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat, Other (Wide Range)
Positive Samples:	C2C12, C6

Immunogen:	A synthetic acetylated peptide around K5 of human Histone H2A (NP_003508.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GRGK Q
Gene ID:	8329
Uniprot:	P0C0S8
Cellular Location:	Chromosome, Nucleus
Calculated MW:	14kDa
Observed MW:	14kDa

Synonyms:

HIST1H2AI, H2A/c, H2AFC

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

UniProt Protein Function:	H2A.1: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H2A family.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p22.1 Cellular Component: nuclear chromatin; nucleus Molecular Function:DNA binding; enzyme binding; protein binding Biological Process: chromatin silencing
NCBI Summary:	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015]
UniProt Code:	P0C0S8
NCBI GenInfo Identifier:	83288406
NCBI Gene ID:	8969
NCBI Accession:	P0C0S8.2
UniProt Secondary Accession:	P0C0S8,P02261, Q2M1R2, Q76PA6,
UniProt Related Accession:	P0C0S8
Molecular Weight:	14kDa
NCBI Full Name:	Histone H2A type 1
NCBI Synonym Full Names:	histone cluster 1 H2A family member g
NCBI Official Symbol:	HIST1H2AG
NCBI Official Synonym Symbols:	H2AG; H2A/p; H2AFP; H2A.1b; pH2A/f
NCBI Protein Information:	histone H2A type 1
UniProt Protein Name:	Histone H2A type 1
UniProt Synonym Protein Names:	Histone H2A/p
UniProt Gene Name:	HIST1H2AG

	Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2A-K5 antibody (CAB15620) at 1:1000 dilution.C2C12 cells and C6 cells were treated by TSA (1 uM) at 37'C for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human breast using H2AK5ac antibody (CAB15620) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using H2AK5ac antibody (CAB15620) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using Acetyl-Histone H2A-K5 antibody (CAB15620) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37'C for 18 hours. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2A-K5 antibody (CAB15620) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37'C for 18 hours. Blue: DAPI for nuclear staining.