Metabolism Antibodies 1
Anti-acetyl-ACC1 Antibody (CAB15606)
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- SKU:
- CAB15606
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-acetyl-ACC1 Antibody |
| Antibody SKU: | CAB15606 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human acetyl-CoA carboxylase alpha. |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, C2C12, C6 |
| Immunogen: | A synthetic peptide of human acetyl-CoA carboxylase alpha. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 31 |
| Uniprot: | Q13085 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 257kDa/259kDa/265kDa/269kDa |
| Observed MW: | 240kDa |
| Synonyms: | ACACA, ACAC, ACACAD, ACC, ACC1, ACCA |
| Background: | Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. |
| UniProt Protein Function: | ACC1: a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Phosphorylation by AMPK or PKA inhibits the enzymatic activity of ACC. ACC-alpha is the predominant isoform in liver, adipocyte and mammary gland. ACC-beta is the major isoform in skeletal muscle and heart. Phosphorylation regulates its activity. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; Ligase; EC 6.4.1.2; EC 6.3.4.14; Lipid Metabolism - fatty acid biosynthesis Chromosomal Location of Human Ortholog: 17q21 Cellular Component: actin cytoskeleton; cytoplasm; cytosol; mitochondrion; nucleolus Molecular Function:acetyl-CoA carboxylase activity; ATP binding; biotin carboxylase activity; metal ion binding; protein binding Biological Process: acetyl-CoA metabolic process; biotin metabolic process; carnitine shuttle; cellular lipid metabolic process; energy reserve metabolic process; fatty acid biosynthetic process; lipid homeostasis; multicellular organismal protein metabolic process; positive regulation of cellular metabolic process; protein homotetramerization; tissue homeostasis; triacylglycerol biosynthetic process; vitamin metabolic process; water-soluble vitamin metabolic process Disease: Acetyl-coa Carboxylase Deficiency |
| NCBI Summary: | Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13085 |
| NCBI GenInfo Identifier: | 118601083 |
| NCBI Gene ID: | 31 |
| NCBI Accession: | Q13085.2 |
| UniProt Secondary Accession: | Q13085,Q6KEV6, Q6XDA8, Q7Z2G8, Q7Z561, Q7Z563, Q7Z564 Q86WB2, Q86WB3, B2RP68, |
| UniProt Related Accession: | Q13085 |
| Molecular Weight: | 269,999 Da |
| NCBI Full Name: | Acetyl-CoA carboxylase 1 |
| NCBI Synonym Full Names: | acetyl-CoA carboxylase alpha |
| NCBI Official Symbol: | ACACA |
| NCBI Official Synonym Symbols: | ACC; ACAC; ACC1; ACCA; ACACAD |
| NCBI Protein Information: | acetyl-CoA carboxylase 1 |
| UniProt Protein Name: | Acetyl-CoA carboxylase 1 |
| UniProt Synonym Protein Names: | ACC-alphaIncluding the following 1 domains:Biotin carboxylase (EC:6.3.4.14 |
| UniProt Gene Name: | ACACA |
| UniProt Entry Name: | ACACA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using acetyl-ACC1 antibody (CAB15606) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of 293T cells using acetyl-ACC1 antibody (CAB15606) at dilution of 1:100. 293T cells were treated by Hydrogen Peroxide (2 nM) at 37'C for 15 minutes after serum-starvation overnight(left). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of C6 cells using acetyl-ACC1 antibody (CAB15606) at dilution of 1:100. C6 cells were treated by Hydrogen Peroxide (2 nM) at 37'C for 15 minutes after serum-starvation overnight(left). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using acetyl-ACC1 antibody (CAB15606) at dilution of 1:100. HeLa cells were treated by Hydrogen Peroxide (2 nM) at 37'C for 15 minutes after serum-starvation overnight(left). Blue: DAPI for nuclear staining. |
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