Immunology Antibodies 2
Anti-ACBD3 Antibody (CAB16568)
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- SKU:
- CAB16568
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-ACBD3 Antibody |
| Antibody SKU: | CAB16568 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human ACBD3 (NP_073572.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, Mouse testis, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human ACBD3 (NP_073572.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAV LNAE RLEV SVDG LTLS PDPE ERPG AEGA PLLP PPLP PPSP PGSG RGPG ASGE QPEP GEAA AGGA AEEA RRLE QRWG FGLE ELYG LALR FFKE KDGK AFHP TYEE KLKL VALH KQVL MGPY NPDT CPEV GFFD VLGN DRRR EWAA LGNM SKED AMVE |
| Gene ID: | 64746 |
| Uniprot: | Q9H3P7 |
| Cellular Location: | Cytoplasmic side, Golgi apparatus membrane, Mitochondrion, Peripheral membrane protein |
| Calculated MW: | 60kDa |
| Observed MW: | 80kDa |
| Synonyms: | ACBD3, GCP60, GOCAP1, GOLPH1, PAP7 |
| Background: | The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is involved in the maintenance of Golgi structure and function through its interaction with the integral membrane protein giantin. It may also be involved in the hormonal regulation of steroid formation. |
| UniProt Protein Function: | ACBD3: Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 1q42.12 Cellular Component: Golgi membrane; Golgi apparatus; membrane; mitochondrion; integral to membrane Molecular Function:protein binding; acyl-CoA binding Biological Process: transport; steroid biosynthetic process |
| NCBI Summary: | The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is involved in the maintenance of Golgi structure and function through its interaction with the integral membrane protein giantin. It may also be involved in the hormonal regulation of steroid formation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9H3P7 |
| NCBI GenInfo Identifier: | 55777312 |
| NCBI Gene ID: | 64746 |
| NCBI Accession: | AAH45533.1 |
| UniProt Secondary Accession: | Q9H3P7,Q5VTJ0, Q6P9F1, Q8IZC5, Q8N4D6, Q9H6U3, B2RB29 |
| UniProt Related Accession: | Q9H3P7 |
| Molecular Weight: | 60,593 Da |
| NCBI Full Name: | Acyl-Coenzyme A binding domain containing 3 |
| NCBI Synonym Full Names: | acyl-CoA binding domain containing 3 |
| NCBI Official Symbol: | ACBD3 |
| NCBI Official Synonym Symbols: | PAP7; GCP60; GOCAP1; GOLPH1 |
| NCBI Protein Information: | Golgi resident protein GCP60; golgi phosphoprotein 1; PKA (RIalpha)-associated protein; PBR- and PKA-associated protein 7; golgi complex associated protein 1, 60kDa; acyl-Coenzyme A binding domain containing 3; peripheral benzodiazepine receptor-associated protein PAP7 |
| UniProt Protein Name: | Golgi resident protein GCP60 |
| UniProt Synonym Protein Names: | Acyl-CoA-binding domain-containing protein 3; Golgi complex-associated protein 1; GOCAP1; Golgi phosphoprotein 1; GOLPH1; PBR- and PKA-associated protein 7; Peripheral benzodiazepine receptor-associated protein PAP7 |
| Protein Family: | Golgi resident protein |
| UniProt Gene Name: | ACBD3 |
| UniProt Entry Name: | GCP60_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ACBD3 Rabbit pAb (CAB16568) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of C6 cells using ACBD3 antibody (CAB16568) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using ACBD3 antibody (CAB16568) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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