Metabolism Antibodies 1

Anti-ACAT1 Antibody (CAB13273)

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SKU:
CAB13273
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-ACAT1 Antibody
Antibody SKU:CAB13273
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human ACAT1 (NP_000010.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SW620, Raji, H460, HepG2, Mouse liver, Mouse testis, Mouse small intestine
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human ACAT1 (NP_000010.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAVL AALL RSGA RSRS PLLR RLVQ EIRY VERS YVSK PTLK EVVI VSAT RTPI GSFL GSLS LLPA TKLG SIAI QGAI EKAG IPKE EVKE AYMG NVLQ GGEG QAPT RQAV LGAG LPIS TPCT TINK VCAS GMKA IMMA SQSL MCGH Q
Gene ID:38
Uniprot:P24752
Cellular Location:Mitochondrion
Calculated MW:42kDa
Observed MW:42kDa
Synonyms:ACAT1, ACAT, MAT, T2, THIL
Background:This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
UniProt Protein Function:ACAT1: Plays a major role in ketone body metabolism. Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD); also known as alpha- methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3- hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. Belongs to the thiolase family.
UniProt Protein Details:

Protein type:Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Lipid Metabolism - fatty acid; Acetyltransferase; Lipid Metabolism - synthesis and degradation of ketone bodies; Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - tryptophan; EC 2.3.1.9; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - butanoate; Mitochondrial

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function:acetyl-CoA C-acetyltransferase activity

Biological Process: branched chain family amino acid catabolic process; ketone body biosynthetic process; ketone body catabolic process

Disease: Alpha-methylacetoacetic Aciduria

NCBI Summary:This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]
UniProt Code:P24752
NCBI GenInfo Identifier:135755
NCBI Gene ID:38
NCBI Accession:P24752.1
UniProt Secondary Accession:P24752,Q96FG8, B2R6H1, G3XAB4,
UniProt Related Accession:P24752
Molecular Weight:17,175 Da
NCBI Full Name:Acetyl-CoA acetyltransferase, mitochondrial
NCBI Synonym Full Names:acetyl-CoA acetyltransferase 1
NCBI Official Symbol:ACAT1  
NCBI Official Synonym Symbols:T2; MAT; ACAT; THIL  
NCBI Protein Information:acetyl-CoA acetyltransferase, mitochondrial
UniProt Protein Name:Acetyl-CoA acetyltransferase, mitochondrial
UniProt Synonym Protein Names:Acetoacetyl-CoA thiolase; T2
UniProt Gene Name:ACAT1  
UniProt Entry Name:THIL_HUMAN
Anti-ACAT1 Antibody (CAB13273)Western blot analysis of extracts of various cell lines, using ACAT1 antibody (CAB13273) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-ACAT1 Antibody (CAB13273)Immunohistochemistry of paraffin-embedded human liver cancer using ACAT1 antibody (CAB13273) at dilution of 1:100 (40x lens).
Anti-ACAT1 Antibody (CAB13273)Immunohistochemistry of paraffin-embedded rat ovary using ACAT1 antibody (CAB13273) at dilution of 1:100 (40x lens).
Anti-ACAT1 Antibody (CAB13273)Immunohistochemistry of paraffin-embedded mouse kidney using ACAT1 antibody (CAB13273) at dilution of 1:100 (40x lens).
Anti-ACAT1 Antibody (CAB13273)Immunofluorescence analysis of C6 cells using ACAT1 antibody (CAB13273) at dilution of 1:100. Blue: DAPI for nuclear staining.
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