Cell Biology Antibodies 2
Anti-ACAP2 Antibody (CAB12127)
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- SKU:
- CAB12127
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ACAP2 Antibody |
| Antibody SKU: | CAB12127 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-160 of human ACAP2 (NP_036419.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | LO2, HeLa, Jurkat, U-87MG, A-549, Mouse brain, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-160 of human ACAP2 (NP_036419.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KAFC VANK QFMN GIRD LAQY SSND AVVE TSLT KFSD SLQE MINF HTIL FDQT QRSI KAQL QNFV KEDL RKFK DAKK QFEK VSEE KENA LVKN AQVQ RNKQ HEVE EATN ILT |
| Gene ID: | 23527 |
| Uniprot: | Q15057 |
| Cellular Location: | Endosome membrane, Peripheral membrane protein |
| Calculated MW: | 88kDa |
| Observed MW: | 115kDa |
| Synonyms: | ACAP2, CENTB2, CNT-B2 |
| Background: |
| UniProt Protein Function: | CENTB2: a member of the centaurin family of ADP-ribosylation factor directed GTPase-activating proteins. Regulates cytoskeletal and membrane trafficking events in the cell periphery. Binding of phosphoinositides to a pleckstrin homology domain may regulate subcellular localization and activity. |
| UniProt Protein Details: | Protein type:GAP; GAP, ARF; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3q29 Cellular Component: endosome membrane; membrane |
| UniProt Code: | Q15057 |
| NCBI GenInfo Identifier: | 39932727 |
| NCBI Gene ID: | 23527 |
| NCBI Accession: | Q15057.3 |
| UniProt Secondary Accession: | Q15057,Q8N5Z8, Q9UQR3, A8K2V4, |
| UniProt Related Accession: | Q15057 |
| Molecular Weight: | 88kDa |
| NCBI Full Name: | Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2 |
| NCBI Synonym Full Names: | ArfGAP with coiled-coil, ankyrin repeat and PH domains 2 |
| NCBI Official Symbol: | ACAP2 |
| NCBI Official Synonym Symbols: | CENTB2; CNT-B2 |
| NCBI Protein Information: | arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2 |
| UniProt Protein Name: | Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2 |
| UniProt Synonym Protein Names: | Centaurin-beta-2; Cnt-b2 |
| Protein Family: | Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein |
| UniProt Gene Name: | ACAP2 |
 | Western blot analysis of extracts of various cell lines, using ACAP2 antibody (CAB12127) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of U2OS cells using ACAP2 Rabbit pAb (CAB12127) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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