Antibody Name:	Anti-ACADVL Antibody
Antibody SKU:	CAB7865
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human ACADVL (NP_000009.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	K-562, LO2, A-549, U-87MG, HeLa, SW620, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human ACADVL (NP_000009.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MQAA RMAA SLGR QLLR LGGG SSRL TALL GQPR PGPA RRPY AGGA AQLA LDKS DSHP SDAL TRKK PAKA ESKS FAVG MFKG QLTT DQVF PYPS VLNE EQTQ FLKE LVEP VSRF FEEV NDPA KNDA LEMV EETT WQGL KELG
Gene ID:	37
Uniprot:	P49748
Cellular Location:	Mitochondrion inner membrane
Calculated MW:	68kDa/70kDa/72kDa
Observed MW:	70kDa

Synonyms:

ACADVL, ACAD6, LCACD, VLCAD

Background:

The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	BAZ1A: Component of the ACF complex, an ATP-dependent chromatin remodeling complex, that regulates spacing of nucleosomes using ATP to generate evenly spaced nucleosomes along the chromatin. The ATPase activity of the complex is regulated by the length of flanking DNA. Also involved in facilitating the DNA replication process. BAZ1A is the accessory, non-catalytic subunit of the complex which can enhance and direct the process provided by the ATPase subunit, SMARCA5, probably through targeting pericentromeric heterochromatin in late S phase. Moves end- positioned nucleosomes to a predominantly central position. May have a role in nuclear receptor-mediated transcription repression. Component of the ACF chromatin remodeling complex that includes BAZ1A and SMARCA5. Additional this complex can form, together with CHRAC1 and POLE1, the histone-fold protein complex, CHRAC. Interacts with NCOR1 (via its RD1 domain); the interaction corepresses a number of NCOR1-regulated genes. Highly expressed in testis and at low or undetectable levels in other tissues analyzed. Belongs to the WAL family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Kinase, protein; Nuclear receptor co-regulator; Protein kinase, Ser/Thr (non-receptor); DNA replication; DNA-binding; ATYPICAL group; BAZ family Chromosomal Location of Human Ortholog: 14q13.2 Cellular Component: nuclear chromosome; chromatin accessibility complex Molecular Function:protein binding; histone acetyltransferase activity; zinc ion binding Biological Process: chromatin remodeling; transcription, DNA-dependent; regulation of transcription, DNA-dependent; DNA-dependent DNA replication
NCBI Summary:	The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	P49748
NCBI GenInfo Identifier:	4557235
NCBI Gene ID:	37
NCBI Accession:	NP_000009
UniProt Secondary Accession:	P49748,O76056, Q8WUL0, B4DEB6, F5H2A9,
UniProt Related Accession:	P49748
Molecular Weight:	68.5 kDa (636aa)
NCBI Full Name:	very long-chain specific acyl-CoA dehydrogenase, mitochondrial isoform 1
NCBI Synonym Full Names:	acyl-CoA dehydrogenase very long chain
NCBI Official Symbol:	ACADVL
NCBI Official Synonym Symbols:	ACAD6; LCACD; VLCAD
NCBI Protein Information:	very long-chain specific acyl-CoA dehydrogenase, mitochondrial
UniProt Protein Name:	Bromodomain adjacent to zinc finger domain protein 1A
UniProt Synonym Protein Names:	ATP-dependent chromatin-remodeling protein; ATP-utilizing chromatin assembly and remodeling factor 1; hACF1; CHRAC subunit ACF1; Williams syndrome transcription factor-related chromatin-remodeling factor 180; WCRF180; hWALp1
Protein Family:	Bromodomain adjacent to zinc finger domain protein
UniProt Gene Name:	BAZ1A
UniProt Entry Name:	BAZ1A_HUMAN

	Western blot analysis of extracts of various cell lines, using ACADVL antibody (CAB7865) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of HeLa cells using ACADVL antibody (CAB7865) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.