Anti-ACADS / SCAD Antibody (CAB20926)

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SKU:
CAB20926
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Plant - Arabidopsis
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-ACADS / SCAD Antibody (CAB20926)
Antibody SKU:CAB20926
Antibody Size:50µL, 100µL
Application:Western blotting, Immunohistochemistry, Immunoprecipitation
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of Human ACADS / SCAD.
Application:Western blotting, Immunohistochemistry, Immunoprecipitation
Recommended Dilution:WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Jurkat, HL-60, Mouse liver, Mouse skeletal muscle, Mouse heart, Rat liver, Rat skeletal muscle, Rat heart
Immunogen:Recombinant protein of Human ACADS / SCAD.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:Mitochondrion matrix
Calculated MW:44kDa
Observed MW:41KDa
Synonyms:ACADS, ACAD3, SCAD
Background:This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms.
Immunoprecipitation analysis of 600ug extracts of Mouse liver cells using 3ug ACADS / SCAD antibody . Western blot was performed from the immunoprecipitate using ACADS / SCAD antibody at a dilition of 1:500.Immunoprecipitation analysis of 600ug extracts of Mouse liver cells using 3ug ACADS / SCAD antibody . Western blot was performed from the immunoprecipitate using ACADS / SCAD antibody at a dilition of 1:500.
Immunohistochemistry of paraffin-embedded rat kidney using ACADS / SCAD Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded rat kidney using ACADS / SCAD Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human colon carcinoma using ACADS / SCAD Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded human colon carcinoma using ACADS / SCAD Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver using ACADS / SCAD Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded human liver using ACADS / SCAD Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Western blot analysis of extracts of various cell lines, using ACADS/SCAD antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Western blot analysis of extracts of various cell lines, using ACADS/SCAD antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
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