Name: Anti-ACADS Antibody (CAB7230)
Brand: Metabolism Antibodies 2
SKU: CAB7230
Availability: OutOfStock

Antibody Name:	Anti-ACADS Antibody
Antibody SKU:	CAB7230
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ACADS (NP_000008.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF-7, HepG2, SKOV3, 293T, HeLa, Mouse liver, Mouse kidney, mouse heart, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ACADS (NP_000008.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAA LLAR ASGP ARRA LCPR AWRQ LHTI YQSV ELPE THQM LLQT CRDF AEKE LFPI AAQV DKEH LFPA AQVK KMGG LGLL AMDV PEEL GGAG LDYL AYAI AMEE ISRG CAST GVIM SVNN SLYL GPIL KFGS KEQK QAWV TPFT SGDK IGCF ALSE PGNG SDAG AAST TARA EGDS WVLN GTKA WITN AWEA SAAV VFAS TDRA LQNK GISA FLVP MPTP GLTL GKKE DKLG IRGS STAN LIFE DCRI PKDS ILGE PGMG
Gene ID:	35
Uniprot:	P16219
Cellular Location:	Mitochondrion matrix
Calculated MW:	44kDa
Observed MW:	44kDa

Synonyms:	ACADS, ACAD3, SCAD
Background:	This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms.

UniProt Protein Function:	ACADS: Defects in ACADS are the cause of acyl-CoA dehydrogenase short-chain deficiency (ACADSD). It is an autosomal recessive disorder resulting in acute acidosis and muscle weakness in infants, and a form of lipid-storage myopathy in adults. Belongs to the acyl-CoA dehydrogenase family.
UniProt Protein Details:	Protein type:EC 1.3.8.1; Mitochondrial; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Oxidoreductase; Carbohydrate Metabolism - butanoate; Lipid Metabolism - fatty acid Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial membrane; nucleus Molecular Function:acyl-CoA dehydrogenase activity; butyryl-CoA dehydrogenase activity; FAD binding; acyl-CoA binding Biological Process: response to starvation; fatty acid beta-oxidation; response to glucocorticoid stimulus; cellular lipid metabolic process; fatty acid beta-oxidation using acyl-CoA dehydrogenase; butyrate catabolic process; protein homotetramerization Disease: Acyl-coa Dehydrogenase, Short-chain, Deficiency Of
NCBI Summary:	This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms. [provided by RefSeq, Oct 2014]
UniProt Code:	P16219
NCBI GenInfo Identifier:	113019
NCBI Gene ID:	35
NCBI Accession:	P16219.1
UniProt Secondary Accession:	P16219,P78331,
UniProt Related Accession:	P16219
Molecular Weight:	412
NCBI Full Name:	Short-chain specific acyl-CoA dehydrogenase, mitochondrial
NCBI Synonym Full Names:	acyl-CoA dehydrogenase, C-2 to C-3 short chain
NCBI Official Symbol:	ACADS
NCBI Official Synonym Symbols:	SCAD; ACAD3
NCBI Protein Information:	short-chain specific acyl-CoA dehydrogenase, mitochondrial; butyryl-CoA dehydrogenase; unsaturated acyl-CoA reductase; acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain; mitochondrial short-chain specific acyl-CoA dehydrogenase
UniProt Protein Name:	Short-chain specific acyl-CoA dehydrogenase, mitochondrial
UniProt Synonym Protein Names:	Butyryl-CoA dehydrogenase
Protein Family:	Short-chain specific acyl-CoA dehydrogenase
UniProt Gene Name:	ACADS
UniProt Entry Name:	ACADS_HUMAN

	Western blot analysis of extracts of various cell lines, using ACADS antibody (CAB7230) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunofluorescence analysis of MCF7 cells using ACADS antibody (CAB7230). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug ACADS antibody (CAB7230). Western blot was performed from the immunoprecipitate using ACADS antibody (CAB7230) at a dilition of 1:1000.