KO Validated Antibodies 2

Anti-ACAD9 Antibody (CAB19942)[KO Validated]

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SKU:
CAB19942
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ACAD9 Antibody [KO Validated]
Antibody SKU:CAB19942
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human ACAD9.
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant protein of human ACAD9.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:28976
Uniprot:Q9H845
Cellular Location:Mitochondrion
Calculated MW:68kDa
Observed MW:69KDa
Anti-ACAD9 Antibody [KO Validated] (CAB19942)Western blot analysis of extracts from normal (control) and ACAD9 knockout (KO) HeLa cells, using ACAD9 antibody (CAB19942) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:ACAD9, NPD002
Background:This gene encodes a member of the acyl-CoA dehydrogenase family. Members of this family of proteins localize to the mitochondria and catalyze the rate-limiting step in the beta-oxidation of fatty acyl-CoA. The encoded protein is specifically active toward palmitoyl-CoA and long-chain unsaturated substrates. Mutations in this gene cause acyl-CoA dehydrogenase family member type 9 deficiency. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:ACAD9: Has a dehydrogenase activity on palmitoyl-CoA (C16:0) and stearoyl-CoA (C18:0). It is three times more active on palmitoyl-CoA then on stearoyl-CoA. Has little activity on octanoyl-CoA (C8:0), butyryl-CoA (C4:0) or isovaleryl-CoA (5:0). Belongs to the acyl-CoA dehydrogenase family.
UniProt Protein Details:

Protein type:EC 1.3.99.-; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: dendrite; mitochondrial inner membrane; mitochondrion; nucleus

Molecular Function:acyl-CoA binding; acyl-CoA dehydrogenase activity; electron carrier activity; FAD binding; protein binding

Biological Process: fatty acid beta-oxidation using acyl-CoA dehydrogenase; lipid homeostasis; mitochondrial respiratory chain complex I assembly

Disease: Acyl-coa Dehydrogenase Family, Member 9, Deficiency Of

NCBI Summary:This gene encodes a member of the acyl-CoA dehydrogenase family. Members of this family of proteins localize to the mitochondria and catalyze the rate-limiting step in the beta-oxidation of fatty acyl-CoA. The encoded protein is specifically active toward palmitoyl-CoA and long-chain unsaturated substrates. Mutations in this gene cause acyl-CoA dehydrogenase family member type 9 deficiency. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Mar 2010]
UniProt Code:Q9H845
NCBI GenInfo Identifier:21361497
NCBI Gene ID:28976
NCBI Accession:
UniProt Secondary Accession:Q9H845,Q8WXX3, D3DNB8,
UniProt Related Accession:Q9H845
Molecular Weight:69kDa
NCBI Full Name:acyl-CoA dehydrogenase family member 9, mitochondrial
NCBI Synonym Full Names:acyl-CoA dehydrogenase family member 9
NCBI Official Symbol:ACAD9  
NCBI Official Synonym Symbols:NPD002  
NCBI Protein Information:acyl-CoA dehydrogenase family member 9, mitochondrial
UniProt Protein Name:Acyl-CoA dehydrogenase family member 9, mitochondrial
Protein Family:Acyl-CoA dehydrogenase family
UniProt Gene Name:ACAD9  
UniProt Entry Name:ACAD9_HUMAN
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