Metabolism Antibodies 3
Anti-ACAA2 Antibody (CAB15778)
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- SKU:
- CAB15778
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-ACAA2 Antibody |
| Antibody SKU: | CAB15778 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ACAA2 (NP_006102.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, A-549, HeLa, Mouse heart, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ACAA2 (NP_006102.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MALL RGVF VVAA KRTP FGAY GGLL KDFT ATDL SEFA AKAA LSAG KVSP ETVD SVIM GNVL QSSS DAIY LARH VGLR VGIP KETP ALTI NRLC GSGF QSIV NGCQ EICV KEAE VVLC GGTE SMSQ APYC VRNV RFGT KLGS DIKL EDSL WVSL TDQH VQLP MAMT AENL AVKH KISR EECD KYAL QSQQ RWKA ANDA GYFN DEMA PIEV KTKK GKQT MQVD EHAR PQTT LEQL QKLP PVFK KDGT VTAG NA |
| Gene ID: | 10449 |
| Uniprot: | P42765 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 41kDa |
| Observed MW: | 50kDa |
| Synonyms: | ACAA2, DSAEC |
| Background: | The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal. |
| UniProt Protein Function: | ACAA2: Abolishes BNIP3-mediated apoptosis and mitochondrial damage. Belongs to the thiolase family. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - valine, leucine and isoleucine degradation; EC 2.3.1.16; Lipid Metabolism - fatty acid; Lipid Metabolism - fatty acid elongation in mitochondria; Transferase Chromosomal Location of Human Ortholog: 18q21.1 Cellular Component: mitochondrial matrix; mitochondrion Molecular Function:acetyl-CoA C-acyltransferase activity; protein binding; RNA binding Biological Process: fatty acid beta-oxidation |
| NCBI Summary: | The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P42765 |
| NCBI GenInfo Identifier: | 167614485 |
| NCBI Gene ID: | 10449 |
| NCBI Accession: | NP_006102.2 |
| UniProt Secondary Accession: | P42765,Q9BUT6, |
| UniProt Related Accession: | P42765 |
| Molecular Weight: | |
| NCBI Full Name: | 3-ketoacyl-CoA thiolase, mitochondrial |
| NCBI Synonym Full Names: | acetyl-CoA acyltransferase 2 |
| NCBI Official Symbol: | ACAA2 |
| NCBI Official Synonym Symbols: | DSAEC |
| NCBI Protein Information: | 3-ketoacyl-CoA thiolase, mitochondrial |
| UniProt Protein Name: | 3-ketoacyl-CoA thiolase, mitochondrial |
| UniProt Synonym Protein Names: | Acetyl-CoA acyltransferase; Beta-ketothiolase; Mitochondrial 3-oxoacyl-CoA thiolase; T1 |
| Protein Family: | 3-ketoacyl-CoA thiolase |
| UniProt Gene Name: | ACAA2 |
 | Western blot analysis of extracts of various cell lines, using ACAA2 antibody (CAB15778) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded Human colon carcinoma using ACAA2 Rabbit pAb (CAB15778) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using ACAA2 antibody (CAB15778) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using ACAA2 antibody (CAB15778) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using ACAA2 antibody (CAB15778) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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