Name: Anti-ABI1 Antibody (CAB16092)
Brand: Immunology Antibodies 2
SKU: CAB16092
Availability: OutOfStock

Antibody Name:	Anti-ABI1 Antibody
Antibody SKU:	CAB16092
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 180-230 of human ABI1 (NP_001012770).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse
Positive Samples:	Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 180-230 of human ABI1 (NP_001012770).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PMSG RGTL GRNT PYKT LEPV KPPT VPND YMTS PARL GSQH SPGR TASL NQR
Gene ID:	10006
Uniprot:	Q8IZP0
Cellular Location:	Cell junction, Cell projection, Cytoplasm, Nucleus, cytoskeleton, filopodium, growth cone, lamellipodium, postsynaptic cell membrane, postsynaptic density, synapse
Calculated MW:	35-55kDa
Observed MW:	55kDa

Synonyms:

ABI1, ABI-1, ABLBP4, E3B1, NAP1BP, SSH3BP, SSH3BP1

Background:

This gene encodes a member of the Abelson-interactor family of adaptor proteins. These proteins facilitate signal transduction as components of several multiprotein complexes, and regulate actin polymerization and cytoskeletal remodeling through interactions with Abelson tyrosine kinases. The encoded protein plays a role in macropinocytosis as a component of the WAVE2 complex, and also forms a complex with EPS8 and SOS1 that mediates signal transduction from Ras to Rac. This gene may play a role in the progression of several malignancies including melanoma, colon cancer and breast cancer, and a t(10;11) chromosomal translocation involving this gene and the MLL gene has been associated with acute myeloid leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 14.

UniProt Protein Function:	Abi-1: an SH3-containing adapter protein that regulates c-Abl-mediated phosphorylation of Mena. Binds the SH3 domains of epidermal growth factor receptor kinase substrate (EPS8) and of spectrin. May play a role in fusion of macropinocytic vesicles. Associated with congenital acute monocytic leukemia.
UniProt Protein Details:	Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 10p11.2 Cellular Component: postsynaptic membrane; growth cone; cytoskeleton; endoplasmic reticulum; lamellipodium; postsynaptic density; intracellular; nucleus; cytosol; cell junction; filopodium Molecular Function:protein binding; cytoskeletal protein binding; protein complex binding; protein tyrosine kinase activator activity Biological Process: negative regulation of cell proliferation; somitogenesis; peptidyl-tyrosine phosphorylation; actin polymerization and/or depolymerization; innate immune response; cell motility; vascular endothelial growth factor receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway
NCBI Summary:	This gene encodes a member of the Abelson-interactor family of adaptor proteins. These proteins facilitate signal transduction as components of several multiprotein complexes, and regulate actin polymerization and cytoskeletal remodeling through interactions with Abelson tyrosine kinases. The encoded protein plays a role in macropinocytosis as a component of the WAVE2 complex, and also forms a complex with EPS8 and SOS1 that mediates signal transduction from Ras to Rac. This gene may play a role in the progression of several malignancies including melanoma, colon cancer and breast cancer, and a t(10;11) chromosomal translocation involving this gene and the MLL gene has been associated with acute myeloid leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 14. [provided by RefSeq, Sep 2011]
UniProt Code:	Q8IZP0
NCBI GenInfo Identifier:	50400546
NCBI Gene ID:	10006
NCBI Accession:	Q8IZP0.4
UniProt Related Accession:	Q8IZP0
Molecular Weight:	55kDa
NCBI Full Name:	Abl interactor 1
NCBI Synonym Full Names:	abl interactor 1
NCBI Official Symbol:	ABI1
NCBI Official Synonym Symbols:	E3B1; ABI-1; ABLBP4; NAP1BP; SSH3BP; SSH3BP1
NCBI Protein Information:	abl interactor 1
UniProt Protein Name:	Abl interactor 1
UniProt Synonym Protein Names:	Abelson interactor 1; Abi-1; Abl-binding protein 4; AblBP4; Eps8 SH3 domain-binding protein; Eps8-binding protein; Nap1-binding protein; Nap1BP; Spectrin SH3 domain-binding protein 1; e3B1
Protein Family:	Abl interactor
UniProt Gene Name:	ABI1
UniProt Entry Name:	ABI1_HUMAN

Western blot analysis of extracts of mouse testis, using ABI1 antibody (CAB16092) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

ABI1 Antibody (PACO06220)

ABI1 Antibody (PACO07533)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)