Cell Biology Antibodies 12

Anti-ABCG5 Antibody (CAB8589)

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SKU:
CAB8589
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ABCG5 Antibody
Antibody SKU:CAB8589
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 350-450 of human ABCG5 (NP_071881.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HL-60
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 350-450 of human ABCG5 (NP_071881.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TLPM VPFK TKDS PGVF SKLG VLLR RVTR NLVR NKLA VITR LLQN LIMG LFLL FFVL RVRS NVLK GAIQ DRVG LLYQ FVGA TPYT GMLN AVNL FPVL RAVS D
Gene ID:64240
Uniprot:Q9H222
Cellular Location:Membrane, Multi-pass membrane protein
Calculated MW:28kDa/72kDa
Observed MW:80kDa
Synonyms:ABCG5, STSL
Background:The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia.
UniProt Protein Function:ABCG5: Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Defects in ABCG5 are a cause of sitosterolemia (STSL); also known as phytosterolemia or shellfish sterolemia. It is a rare autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Sitosterolemia patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease. Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
UniProt Protein Details:

Protein type:Membrane protein, integral; Transporter, ABC family; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: apical plasma membrane; plasma membrane; ATP-binding cassette (ABC) transporter complex; receptor complex

Molecular Function:protein binding; protein heterodimerization activity; cholesterol transporter activity; ATPase activity; ATP binding

Biological Process: response to drug; negative regulation of cholesterol absorption; cholesterol homeostasis; cholesterol absorption; cholesterol efflux; response to ionizing radiation; excretion; transmembrane transport; response to nutrient; sterol transport

Disease: Sitosterolemia

NCBI Summary:The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia. [provided by RefSeq, Jul 2008]
UniProt Code:Q9H222
NCBI GenInfo Identifier:17432917
NCBI Gene ID:64240
NCBI Accession:Q9H222.1
UniProt Secondary Accession:Q9H222,Q99PE8,
UniProt Related Accession:Q9H222
Molecular Weight:28,480 Da
NCBI Full Name:ATP-binding cassette sub-family G member 5
NCBI Synonym Full Names:ATP-binding cassette, sub-family G (WHITE), member 5
NCBI Official Symbol:ABCG5  
NCBI Official Synonym Symbols:STSL  
NCBI Protein Information:ATP-binding cassette sub-family G member 5; sterolin 1; sterolin-1; ATP-binding cassette, subfamily G, member 5
UniProt Protein Name:ATP-binding cassette sub-family G member 5
UniProt Synonym Protein Names:Sterolin-1
Protein Family:ABC transporter G family
UniProt Gene Name:ABCG5  
UniProt Entry Name:ABCG5_HUMAN
Anti-ABCG5 Polyclonal Antibody (CAB8589)Western blot analysis of extracts of HL-60 cells, using ABCG5 antibody (CAB8589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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