Name: Anti-ABCG4 Antibody (CAB14596)
Brand: Signal Transduction Antibodies 1
SKU: CAB14596
Availability: OutOfStock

Antibody Name:	Anti-ABCG4 Antibody
Antibody SKU:	CAB14596
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 270-370 of human ABCG4 (NP_071452.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, A-549, U-937, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 270-370 of human ABCG4 (NP_071452.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KLYI LSQG QCIF KGVV TNLI PYLK GLGL HCPT YHNP ADFI IEVA SGEY GDLN PMLF RAVQ NGLC AMAE KKSS PEKN EVPA PCPP CPPE VDPI ESHT FATS T
Gene ID:	64137
Uniprot:	Q9H172
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	60kDa/64kDa/65kDa/71kDa
Observed MW:	72kDa

Synonyms:

ABCG4, WHITE2

Background:

The protein encoded by this gene is a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). The encoded protein is a member of the White subfamily and plays an important role in cellular cholesterol homeostasis. This protein functions as either a homodimer or as a heterodimer with another ABC subfamily protein such as ABCG1.

UniProt Protein Function:	ABCG4: May be involved in macrophage lipid homeostasis. Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Transporter; Membrane protein, integral; Transporter, ABC family Chromosomal Location of Human Ortholog: 11q23.3 Cellular Component: integral to membrane; plasma membrane Molecular Function:protein binding; protein homodimerization activity; protein heterodimerization activity; cholesterol transporter activity; ATPase activity; ATP binding Biological Process: metabolic process; cholesterol efflux; transmembrane transport
NCBI Summary:	The protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily and is expressed predominantly in liver tissue. The function has not yet been determined but may involve cholesterol transport. Alternate splice variants have been described but their full length sequences have not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9H172
NCBI GenInfo Identifier:	17432915
NCBI Gene ID:	64137
NCBI Accession:	Q9H172.2
UniProt Secondary Accession:	Q9H172,Q8WWH0, Q8WWH1, Q8WWH2, A8K1B5,
UniProt Related Accession:	Q9H172
Molecular Weight:	646
NCBI Full Name:	ATP-binding cassette sub-family G member 4
NCBI Synonym Full Names:	ATP-binding cassette, sub-family G (WHITE), member 4
NCBI Official Symbol:	ABCG4
NCBI Official Synonym Symbols:	WHITE2
NCBI Protein Information:	ATP-binding cassette sub-family G member 4; putative ABC transporter; ATP-binding cassette, subfamily G, member 4
UniProt Protein Name:	ATP-binding cassette sub-family G member 4
Protein Family:	ABC transporter G family
UniProt Gene Name:	ABCG4
UniProt Entry Name:	ABCG4_HUMAN

Western blot analysis of extracts of various cell lines, using ABCG4 antibody (CAB14596) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

ABCG4 Antibody (PACO07528)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)