Signal Transduction Antibodies 3

Anti-ABCG2 Antibody (CAB5661)

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SKU:
CAB5661
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-ABCG2 Antibody
Antibody SKU:CAB5661
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 260-395 of human ABCG2 (NP_004818.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:COS-7, A-549, Mouse liver, Mouse spleen, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 260-395 of human ABCG2 (NP_004818.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ASGR LMFH GPAQ EALG YFES AGYH CEAY NNPA DFFL DIIN GDST AVAL NREE DFKA TEII EPSK QDKP LIEK LAEI YVNS SFYK ETKA ELHQ LSGG EKKK KITV FKEI SYTT SFCH QLRW VSKR SFKN LLGN PQAS
Gene ID:9429
Uniprot:Q9UNQ0
Cellular Location:Cell membrane, Mitochondrion membrane, Multi-pass membrane protein
Calculated MW:67kDa/72kDa
Observed MW:72kDa
Synonyms:ABC15, ABCP, BCRP, BCRP1, BMDP, CD338, CDw338, EST157481, GOUT1, MRX, MXR, MXR-1, MXR1, UAQTL1, ABCG2
Background:The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:ABCG2: Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP- dependent increase in the efflux of rhodamine 123. Monomer or homodimer; disulfide-linked. Up-regulated in brain tumors. Highly expressed in placenta. Low expression in small intestine, liver and colon. Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Transporter; Transporter, ABC family; Membrane protein, multi-pass; Transporter, iron

Chromosomal Location of Human Ortholog: 4q22

Cellular Component: nucleoplasm; mitochondrial membrane; plasma membrane; integral to membrane; nucleus

Molecular Function:protein binding; protein homodimerization activity; transporter activity; ATPase activity, coupled to transmembrane movement of substances; heme transporter activity; ATP binding; xenobiotic-transporting ATPase activity

Biological Process: response to drug; heme transport; urate metabolic process; transport; cellular iron ion homeostasis; xenobiotic transport; multidrug transport; transmembrane transport

Disease: Blood Group, Junior System; Uric Acid Concentration, Serum, Quantitative Trait Locus 1

NCBI Summary:The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
UniProt Code:Q9UNQ0
NCBI GenInfo Identifier:67462103
NCBI Gene ID:9429
NCBI Accession:Q9UNQ0.3
UniProt Secondary Accession:Q9UNQ0,O95374, Q4W5I3, Q53ZQ1, Q569L4, Q5YLG4, Q86V64 Q8IX16, Q96LD6, Q96TA8, A0A1W3, A8K1T5,
UniProt Related Accession:Q9UNQ0
Molecular Weight:72,314 Da
NCBI Full Name:ATP-binding cassette sub-family G member 2
NCBI Synonym Full Names:ATP-binding cassette, sub-family G (WHITE), member 2
NCBI Official Symbol:ABCG2  
NCBI Official Synonym Symbols:MRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CD338; GOUT1; CDw338; UAQTL1; EST157481  
NCBI Protein Information:ATP-binding cassette sub-family G member 2; urate exporter; ABC transporter; placenta specific MDR protein; breast cancer resistance protein; ATP-binding cassette transporter G2; mitoxantrone resistance-associated protein; placenta-specific ATP-binding cassette transporter; multi drug resistance efflux transport ATP-binding cassette sub-family G (WHITE) member 2
UniProt Protein Name:ATP-binding cassette sub-family G member 2
UniProt Synonym Protein Names:Breast cancer resistance protein; CDw338; Mitoxantrone resistance-associated protein; Placenta-specific ATP-binding cassette transporter; Urate exporter
UniProt Gene Name:ABCG2  
UniProt Entry Name:ABCG2_HUMAN
Anti-ABCG2 Antibody (CAB5661)Western blot analysis of extracts of various cell lines, using ABCG2 antibody (CAB5661) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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