Signal Transduction Antibodies 3

Anti-ABCC8 Antibody (CAB8456)

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SKU:
CAB8456

Description

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Antibody Name:Anti-ABCC8 Antibody
Antibody SKU:CAB8456
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 700-1000 of human ABCC8 (NP_000343.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 700-1000 of human ABCC8 (NP_000343.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TIRI PRGQ LTMI VGQV GCGK SSLL LAAL GEMQ KVSG AVFW SSLP DSEI GEDP SPER ETAT DLDI RKRG PVAY ASQK PWLL NATV EENI IFES PFNK QRYK MVIE ACSL QPDI DILP HGDQ TQIG ERGI NLSG GQRQ RISV ARAL YQHA NVVF LDDP FSAL DIHL SDHL MQAG ILEL LRDD KRTV VLVT HKLQ YLPH ADWI IAMK DGTI QREG TLKD FQRS ECQL FEHW KTLM NRQD QELE KETV TERK ATEP PQGL SRAM SSRD GLLQ DEEE EEEE AAES EEDD NLSS MLHQ RAEI PWRA C
Gene ID:6833
Uniprot:Q09428
Cellular Location:Membrane, Multi-pass membrane protein
Calculated MW:5kDa/176kDa/177kDa
Observed MW:177kDa
Synonyms:ABCC8, ABC36, HHF1, HI, HRINS, MRP8, PHHI, SUR, SUR1, SUR1delta2, TNDM2
Background:The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a modulator of ATP-sensitive potassium channels and insulin release. Mutations and deficiencies in this protein have been observed in patients with hyperinsulinemic hypoglycemia of infancy, an autosomal recessive disorder of unregulated and high insulin secretion. Mutations have also been associated with non-insulin-dependent diabetes mellitus type II, an autosomal dominant disease of defective insulin secretion. Alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:ABCC8: a multi-pass membrane protein subunit of the beta-cell ATP-sensitive potassium channel (KATP). Regulator of ATP-sensitive K(+) channels and insulin release. Associates with Kir6.2. Defects in ABCC8 are a cause of persistent hyperinsulinemic hypoglycemia of infancy (PHHI) [MIM:601820, 256450], and may contribute to non-insulin-dependent diabetes mellitus (NIDDM) in Northern European Caucasians. Two alternatively spliced human isoforms have been described.
UniProt Protein Details:

Protein type:Transporter, ABC family; Membrane protein, integral; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: voltage-gated potassium channel complex; plasma membrane

Molecular Function:ATPase activity, coupled to transmembrane movement of substances; sulfonylurea receptor activity; potassium ion transmembrane transporter activity; ATP binding

Biological Process: synaptic transmission; carbohydrate metabolic process; energy reserve metabolic process; signal transduction; regulation of insulin secretion; transmembrane transport; potassium ion transport

Disease: Hyperinsulinemic Hypoglycemia, Familial, 1; Diabetes Mellitus, Transient Neonatal, 2; Diabetes Mellitus, Permanent Neonatal; Hypoglycemia, Leucine-induced; Diabetes Mellitus, Noninsulin-dependent

NCBI Summary:The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a modulator of ATP-sensitive potassium channels and insulin release. Mutations and deficiencies in this protein have been observed in patients with hyperinsulinemic hypoglycemia of infancy, an autosomal recessive disorder of unregulated and high insulin secretion. Mutations have also been associated with non-insulin-dependent diabetes mellitus type II, an autosomal dominant disease of defective insulin secretion. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]
UniProt Code:Q09428
NCBI GenInfo Identifier:311033501
NCBI Gene ID:6833
NCBI Accession:Q09428.6
UniProt Secondary Accession:Q09428,O75948, Q16583, A6NMX8, E3UYX6,
UniProt Related Accession:Q09428
Molecular Weight:5,468 Da
NCBI Full Name:ATP-binding cassette sub-family C member 8
NCBI Synonym Full Names:ATP-binding cassette, sub-family C (CFTR/MRP), member 8
NCBI Official Symbol:ABCC8  
NCBI Official Synonym Symbols:HI; SUR; HHF1; MRP8; PHHI; SUR1; ABC36; HRINS; TNDM2; SUR1delta2  
NCBI Protein Information:ATP-binding cassette sub-family C member 8; sulfonylurea receptor 1; sulfonylurea receptor (hyperinsulinemia); ATP-binding cassette transporter sub-family C member 8
UniProt Protein Name:ATP-binding cassette sub-family C member 8
UniProt Synonym Protein Names:Sulfonylurea receptor 1
Protein Family:ABC transporter C family
UniProt Gene Name:ABCC8  
UniProt Entry Name:ABCC8_HUMAN
Anti-ABCC8 Polyclonal Antibody (CAB8456)Western blot analysis of extracts of mouse pancreas, using ABCC8 antibody (CAB8456) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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Additional Information

Product Type:
Antibody
Applications:
WB
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
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